Results 1 to 10 of about 148,581 (265)

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH [PDF]

open access: yesMolecules, 2019
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome.
Tianheng Ren   +9 more
doaj   +2 more sources

The use of oligonucleotide probes for meningococcal serotype characterization [PDF]

open access: yesRevista do Instituto de Medicina Tropical de São Paulo, 1998
In the present study we examine the potential use of oligonucleotide probes to characterize Neisseria meningitidis serotypes without the use of monoclonal antibodies (MAbs).
SACCHI Claudio Tavares   +6 more
doaj   +4 more sources

PCR detection of Plasmodium falciparum by oligonucleotide probes

open access: yesMolecular and Cellular Probes, 1990
Abstract PCR experiments using rRNA sequence-specific oligonucleotides were used to detect Plasmodium falciparum DNA and Plasmodium vivax DNA from cultures and blood samples.
Isabelle Hatin, L D'Auriol
exaly   +3 more sources

An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes. [PDF]

open access: yesPLoS ONE
A comprehensive and user-friendly method for evaluating recognition of double-stranded DNA (dsDNA) targets by oligonucleotide-based probes is presented. Thus, dsDNA-targeting probes such as single-stranded locked nucleic acids (LNAs) and double-stranded ...
Michaela E Everly   +3 more
doaj   +2 more sources

Caspase-Activated Oligonucleotide Probe [PDF]

open access: yesBioconjugate Chemistry, 2020
Light-activated ("caged") oligonucleotides provide a strategy for modulating the activity of antisense oligos, siRNA, miRNA, aptamers, DNAzymes, and mRNA-capturing probes with high spatiotemporal resolution. However, the near-UV and visible wavelengths that promote these bond-breaking reactions poorly penetrate living tissue, which limits some ...
Linlin Yang   +2 more
openaire   +2 more sources

Universal oligonucleotide microarray for sub-typing of Influenza A virus. [PDF]

open access: yesPLoS ONE, 2011
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9).
Vladimir A Ryabinin   +5 more
doaj   +1 more source

Prider: multiplexed primer design using linearly scaling approximation of set coverage

open access: yesBMC Bioinformatics, 2022
Background Designing oligonucleotide primers and probes is one of the key steps of various laboratory experiments such as multiplexed PCR or digital multiplexed ligation assays.
Niina Smolander   +2 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

open access: yesPLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook   +6 more
doaj   +1 more source

Design and analysis of mismatch probes for long oligonucleotide microarrays

open access: yesBMC Genomics, 2008
Background Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this ...
Van Nostrand Joy D   +3 more
doaj   +1 more source

ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY

open access: yesActa Agriculturae Slovenica, 1998
In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow.
Katarina TEPŠIČ, G. AVGUŠTIN
doaj   +1 more source

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