Results 1 to 10 of about 222,321 (300)

Design and analysis of mismatch probes for long oligonucleotide microarrays [PDF]

BMC Genomics, 2008
Background Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this ...
Van Nostrand Joy D, He Zhili, Deng Ye, Zhou Jizhong   +3 more
doaj   +3 more sources

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH [PDF]

Molecules, 2019
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome.
Tianheng Ren, Maojie He, Zixin Sun, Feiquan Tan, Peigao Luo, Zongxiang Tang, Shulan Fu, Benju Yan, Zhenglong Ren, Zhi Li   +9 more
doaj   +2 more sources

An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes. [PDF]

PLoS ONE
A comprehensive and user-friendly method for evaluating recognition of double-stranded DNA (dsDNA) targets by oligonucleotide-based probes is presented. Thus, dsDNA-targeting probes such as single-stranded locked nucleic acids (LNAs) and double-stranded ...
Michaela E Everly, Peter J Wieber, Ibrahim Al Janabi, Patrick J Hrdlicka   +3 more
doaj   +2 more sources

A novel colorimetric DNA sensor for rapid detection of feline panleukopenia virus using ZnFe2O4 nanoparticles and TMB modulation [PDF]

Scientific Reports
A novel colorimetric genosensor was developed for the detection of feline panleukopenia virus (FPV) DNA, based on the peroxidase-like activity of ZnFe2O4 (ZFO) nanoparticles.
Zahra Abdoli, Hamid Staji, Zohreh Bahrami   +2 more
doaj   +2 more sources

Universal oligonucleotide microarray for sub-typing of Influenza A virus. [PDF]

PLoS ONE, 2011
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9).
Vladimir A Ryabinin, Elena V Kostina, Galiya A Maksakova, Alexander A Neverov, Konstantin M Chumakov, Alexander N Sinyakov   +5 more
doaj   +1 more source

Prider: multiplexed primer design using linearly scaling approximation of set coverage

BMC Bioinformatics, 2022
Background Designing oligonucleotide primers and probes is one of the key steps of various laboratory experiments such as multiplexed PCR or digital multiplexed ligation assays.
Niina Smolander, Timothy R. Julian, Manu Tamminen   +2 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

PLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook, Chi-Kin Chan, Paul Jorgensen, Troy Ketela, Daniel So, Mike Tyers, Chi-Yip Ho   +6 more
doaj   +1 more source

Two new rapid SNP-typing methods for classifying Mycobacterium tuberculosis complex into the main phylogenetic lineages [PDF]

, 2012
There is increasing evidence that strain variation in Mycobacterium tuberculosis complex (MTBC) might influence the outcome of tuberculosis infection and disease.
A Deshpande, AC Schürch, AC Schürch, AG Tsolaki, BC de Jong, BC de Jong, Bijaya Malla, C Allix-Béguec, C Bouakaze, C Bouakaze, C Demay, CM Sassetti, CM Sassetti, CRE McEvoy, D Portevin, D Yeboah-Manu, DA Milner Jr, David Stucki, Dorothy Yeboah-Manu, E Abadia, ES Click, G Thwaites, H Yesilkaya, I Comas, I Comas, I Filliol, I Filliol, I Parwati, Igor Mokrousov, IL Bergval, Iñaki Comas, J Kamerbeek, J Song, J Zhi, J Zhi, JD van Embden, JP Schouten, Julia Feldmann, K Okonechnikov, K Tamura, L Baker, L Fenner, L Fenner, L Flores, L Kaderali, L Wang, LE Espinosa de los Monteros, LS Cowan, Lukas Fenner, M Ben Shabat, M Caws, M Caws, M Coscolla, M Kato-Maeda, M Kato-Maeda, Mireia Coscollà, MM Gutacker, MP Nicol, NH Smith, O Mestre, P Keim, P Supply, P Supply, PM Groenen, PM Holland, PM Vallone, R Hershberg, S Gagneux, S Homolka, S Kim, S Sreevatsan, S-H Lee, SA Dunbar, SD Bentley, Sebastien Gagneux, Simon Hostettler, Sonia Borrell, ST Cole, T Pearson, TA Halse, Thembela Huna, U Nübel, WP Maddison   +82 more
core   +13 more sources

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY

Acta Agriculturae Slovenica, 1998
In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow.
Katarina TEPŠIČ, G. AVGUŠTIN
doaj   +1 more source