Results 1 to 10 of about 152,205 (267)

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH [PDF]

Molecules, 2019
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome.
Tianheng Ren, Maojie He, Zixin Sun, Feiquan Tan, Peigao Luo, Zongxiang Tang, Shulan Fu, Benju Yan, Zhenglong Ren, Zhi Li   +9 more
doaj   +2 more sources

Universal oligonucleotide microarray for sub-typing of Influenza A virus. [PDF]

PLoS ONE, 2011
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9).
Vladimir A Ryabinin, Elena V Kostina, Galiya A Maksakova, Alexander A Neverov, Konstantin M Chumakov, Alexander N Sinyakov   +5 more
doaj   +1 more source

Prider: multiplexed primer design using linearly scaling approximation of set coverage

BMC Bioinformatics, 2022
Background Designing oligonucleotide primers and probes is one of the key steps of various laboratory experiments such as multiplexed PCR or digital multiplexed ligation assays.
Niina Smolander, Timothy R. Julian, Manu Tamminen   +2 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

PLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook, Chi-Kin Chan, Paul Jorgensen, Troy Ketela, Daniel So, Mike Tyers, Chi-Yip Ho   +6 more
doaj   +1 more source

Design and analysis of mismatch probes for long oligonucleotide microarrays

BMC Genomics, 2008
Background Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this ...
Van Nostrand Joy D, He Zhili, Deng Ye, Zhou Jizhong   +3 more
doaj   +1 more source

ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY

Acta Agriculturae Slovenica, 1998
In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow.
Katarina TEPŠIČ, G. AVGUŠTIN
doaj   +1 more source

LUEGO: a cost and time saving gel shift procedure

BioTechniques, 2011
We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach.
Nicolas Jullien, Jean-Paul Herman
doaj   +1 more source

Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

BioTechniques, 2015
Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha, Brian Beliveau, Kassandra Semrau, Donald Schwartz, Chao-ting Wu, Erdogan Gulari, Jean-Marie Rouillard   +6 more
doaj   +1 more source

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

Molecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

Fabrication of DNA Microarrays Using Unmodified Oligonucleotide Probes

BioTechniques, 2001
Microarrays printed on glass slides are often constructed by covalently linking oligonucleotide probes to a derivatized surface. These procedures typically require relatively expensive amine- or thiol-modified oligonucleotide probes that add considerable
Douglas Ruben Call, Darrell P. Chandler, Fred Brockman   +2 more
doaj   +1 more source