Results 11 to 20 of about 152,205 (267)
Caspase-Activated Oligonucleotide Probe [PDF]
Bioconjugate Chemistry, 2020 Light-activated ("caged") oligonucleotides provide a strategy for modulating the activity of antisense oligos, siRNA, miRNA, aptamers, DNAzymes, and mRNA-capturing probes with high spatiotemporal resolution. However, the near-UV and visible wavelengths that promote these bond-breaking reactions poorly penetrate living tissue, which limits some ...
Linlin Yang +2 more
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BioTechniques, 1999 The use of thermodynamic parameters for the calculation of oligonucleotide duplex stability provides the best estimates of oligonucleotide melting temperatures (Tm). Such estimates can be used for evidence-based design of molecular biological experiments
Ekkehard Schütz, Nicolas von Ahsen
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ROSO: optimizing oligonucleotide probes for microarrays [PDF]
Bioinformatics, 2004 Abstract Summary: ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. Availability: The program is available on the internet.
Nancie Reymond +5 more
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Molecules, 2017 Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-
Zhiqiang Xiao +4 more
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On-chip hybridization kinetics for optimization of gene expression experiments
BioTechniques, 2008 DNA microarray technology is a powerful tool for getting an overview of gene expression in biological samples. Although the successful use of microarray-based expression analysis was demonstrated in a number of applications, the main problem with this ...
Elena Khomyakova +7 more
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BioTechniques, 2006 A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single ...
Cesar E. Guerra
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Development of a microfluidic biosensor for the diagnostics and typing of Mycobacterium Tuberculosis [PDF]
Клиническая практика, 2021 Background. Despite on the general trend towards decreasing the incidence of newly diagnosed active forms of tuberculosis, the situation with spreading of this disease in Russian Federation remains extremely tense.
Tatiana V. Mitko +7 more
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Sensors, 2010 The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley +5 more
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Development, characterization and experimental validation of a cultivated sunflower (Helianthus annuus L.) gene expression oligonucleotide microarray. [PDF]
PLoS ONE, 2012 Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences.
Paula Fernandez +17 more
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Protein detection using oligonucleotide probes [PDF]
Nucleic Acids Symposium Series, 2009 We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical reaction and indicates the presence of the target protein ...
Aya, Shibata +4 more
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