Results 11 to 20 of about 222,321 (300)
PROBER: oligonucleotide FISH probe design software [PDF]
Bioinformatics, 2006 PROBER is an oligonucleotide primer design software application that designs multiple primer pairs for generating PCR probes useful for fluorescence in situ hybridization (FISH). PROBER generates Tiling Oligonucleotide Probes (TOPs) by masking repetitive
Grubor, V. +11 more
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Caspase-Activated Oligonucleotide Probe [PDF]
Bioconjugate Chemistry, 2020 Light-activated ("caged") oligonucleotides provide a strategy for modulating the activity of antisense oligos, siRNA, miRNA, aptamers, DNAzymes, and mRNA-capturing probes with high spatiotemporal resolution. However, the near-UV and visible wavelengths that promote these bond-breaking reactions poorly penetrate living tissue, which limits some ...
Linlin Yang +2 more
openaire +2 more sources
Protein detection using oligonucleotide probes [PDF]
Nucleic Acids Symposium Series, 2009 We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical reaction and indicates the presence of the target protein ...
Aya, Shibata +4 more
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LUEGO: a cost and time saving gel shift procedure
BioTechniques, 2011 We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach.
Nicolas Jullien, Jean-Paul Herman
doaj +1 more source
Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays [PDF]
, 2008 Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis.
Baranowski, Eric +4 more
core +3 more sources
BioTechniques, 2015 Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha +6 more
doaj +1 more source
Mixed metal nanoparticle assembly and the effect on surface enhanced raman scattering [PDF]
, 2010 Here we report the assembly of mixed metal nanoparticles using an oligonucleotide-templated approach. Substitution of one of the gold nanoparticle probes with an analagous silver probe to produce a hetero-metal duplex permitted surface enhanced Raman ...
Alivisatos +16 more
core +1 more source
ROSO: optimizing oligonucleotide probes for microarrays [PDF]
Bioinformatics, 2004 Abstract Summary: ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. Availability: The program is available on the internet.
Nancie, Reymond +5 more
openaire +2 more sources
Molecules, 2018 Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
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Fabrication of DNA Microarrays Using Unmodified Oligonucleotide Probes
BioTechniques, 2001 Microarrays printed on glass slides are often constructed by covalently linking oligonucleotide probes to a derivatized surface. These procedures typically require relatively expensive amine- or thiol-modified oligonucleotide probes that add considerable
Douglas Ruben Call +2 more
doaj +1 more source