Results 21 to 30 of about 152,205 (267)
BioTechniques, 1998 The FastTag™ Nucleic Acid Labeling System couples haptens, fluorochromes or affinity ligands to any nucleic acid by attaching a universal, photo- or heat-activatable moiety to which any sulfhydryl-reactive compound can be linked.
Steven G. Daniel +3 more
doaj +1 more source
ProbeMaker: an extensible framework for design of sets of oligonucleotide probes
BMC Bioinformatics, 2005 Background Procedures for genetic analyses based on oligonucleotide probes are powerful tools that can allow highly parallel investigations of genetic material.
Nilsson Mats +2 more
doaj +1 more source
Oligonucleotide probes for mutants streptococci
Molecular and Cellular Probes, 1994 Oligodeoxyribonucleotide probes complementary to hypervariable regions of the 16S ribosomal RNA were designed for specificity toward Streptococcus mutans and Streptococcus sobrinus. The probes were tested for specificity and sensitivity by hybridization with nucleic acid from over 100 mutans and non-mutans oral streptococci and other common ...
G A, Cangelosi +4 more
openaire +2 more sources
BMC Bioinformatics, 2010 Background Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design.
Chen Tsute, Høvik Hedda
doaj +1 more source
Generation of Amplifiable Genome-Specific Oligonucleotide Probes and Libraries
BioTechniques, 2002 Here we describe a process for the generation of oligonucleotide libraries representative of a given nucleic acid. Starting from a random pool of DNA oligonucleotides, the technique selects only those that hybridize to the nucleic acid template.
Ivan Brukner +2 more
doaj +1 more source
Oligonucleotide microarray analysis of aminoallyl-labeled cDNA targets from linear RNA amplification
BioTechniques, 2004 Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe ...
Pal Kaposi-Novak +4 more
doaj +1 more source
Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization
BioTechniques, 2003 Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products.
V. Abécassis +6 more
doaj +1 more source
Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Cells, 2021 Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Molecular probe technology detects bacteria without culture
BMC Microbiology, 2012 Background Our ultimate goal is to detect the entire human microbiome, in health and in disease, in a single reaction tube, and employing only commercially available reagents.
Hyman Richard W +9 more
doaj +1 more source