Results 21 to 30 of about 222,321 (300)

Spreadsheet Software for Thermodynamic Melting Point Prediction of Oligonucleotide Hybridization with and without Mismatches

BioTechniques, 1999
The use of thermodynamic parameters for the calculation of oligonucleotide duplex stability provides the best estimates of oligonucleotide melting temperatures (Tm). Such estimates can be used for evidence-based design of molecular biological experiments
Ekkehard Schütz, Nicolas von Ahsen
doaj   +1 more source

Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts [PDF]

, 2009
A microarray spotted with 369 different 16S rRNA gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. The microarray was tested with pure cultures, and of the 30,258 individual probe-
A Alfreider, A Loy, A Loy, BEE Buchholz-Cleven, Brigitte A. Knapp, CJF Braak ter, D Guschin, D-Y Lee, DA Stahl, DM Barrett, EW Alm, F Schwieger, F Wintzingerode von, G Muyzer, G Taroncher-Oldenburg, G Varani, H Daims, H Sanguin, H Urakawa, HAJ Hoitink, HAJ Hoitink, HAJ Hoitink, Heribert Insam, HM Dionisi, IH Franke-Whittle, IH Franke-Whittle, Ingrid H. Franke-Whittle, J Peplies, J Ryckeboer, Jacques Fuchs, JG Fuchs, K Nakasaki, K-H Kim, KJ Breslauer, L Bodrossy, L Bodrossy, L Ludwig, M Blanc, M Danon, M Hogardt, MA Elorrieta, MT Suzuki, N Stralis-Pavese, P Legendre, P Schloss, PM Dees, R Amann, Ruediger Kaufmann, S Haruta, SF Altschul, SM Tiquia, SM Tiquia, T Beffa, W-T Liu, Y Koizumi   +54 more
core   +1 more source

Oligonucleotides and ND-FISH Displaying Different Arrangements of Tandem Repeats and Identification of Dasypyrum villosum Chromosomes in Wheat Backgrounds

Molecules, 2017
Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-
Zhiqiang Xiao, Shuyao Tang, Ling Qiu, Zongxiang Tang, Shulan Fu   +4 more
doaj   +1 more source

Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensor [PDF]

, 2005
The steady rise of observations of harmful or toxic algal blooms throughout the world in the past decades constitute a menace for coastal ecosystems and human interests.
Huljic, S., Lange, M., Medlin, Linda, Metfies, Katja   +3 more
core   +1 more source

On-chip hybridization kinetics for optimization of gene expression experiments

BioTechniques, 2008
DNA microarray technology is a powerful tool for getting an overview of gene expression in biological samples. Although the successful use of microarray-based expression analysis was demonstrated in a number of applications, the main problem with this ...
Elena Khomyakova, Mikheil A. Livshits, Marie-Caroline Steinhauser, Luce Dauphinot, Sylvia Cohen-Kaminsky, Jean Rossier, Françoise Soussaline, Marie-Claude Potier   +7 more
doaj   +1 more source

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot, A. E. Pozhitkov, A. Halperin, A. Harrison, A. Ott, Amend, B. M. Pettitt, Berger, Binder, Binder, Binder, Binder, Bolstad, Bullard, Burden, Burden, C. Gibas, C. J. Burden, Chou, Chou, Czypionka, D. P. Kreil, D. Tautz, E. Carlon, Fasold, Fasold, Fiche, Fuchs, H. Binder, Halperin, Harr, Harrison, He, Heim, Held, Hooyberghs, Huettel, Iltumur, Irizarry, Irizarry, Irizarry, Irving, J. Hooyberghs, Kane, L. J. Gamble, Lee, Lee, Letowski, Li, Liebich, Lockhart, Luebke, Marshall, Matveeva, Mueckstein, Mulders, Naiser, Naiser, P. A. Noble, Pingel, Pozhitkov, R. Levicky, Relogio, Rouillard, Tanaka, Trapp, Upton, Vainrub, Wodicka, Yu, Zhang   +70 more
core   +3 more sources

Oligonucleotide probes for mutants streptococci

Molecular and Cellular Probes, 1994
Oligodeoxyribonucleotide probes complementary to hypervariable regions of the 16S ribosomal RNA were designed for specificity toward Streptococcus mutans and Streptococcus sobrinus. The probes were tested for specificity and sensitivity by hybridization with nucleic acid from over 100 mutans and non-mutans oral streptococci and other common ...
G A, Cangelosi, J M, Iversen, Y, Zuo, T K, Oswald, R J, Lamont   +4 more
openaire   +2 more sources

Error, reproducibility and sensitivity : a pipeline for data processing of Agilent oligonucleotide expression arrays [PDF]

, 2010
Background Expression microarrays are increasingly used to obtain large scale transcriptomic information on a wide range of biological samples. Nevertheless, there is still much debate on the best ways to process data, to design experiments and analyse ...
Bowen, Helen C., Chain, B. M., Hammond, John P., Noursadeghi, Mahdad, Posch, Wilfried, Rasaiyaah, Jane, Tsang, Jhen   +6 more
core   +2 more sources

Single-Tube Genotyping without Oligonucleotide Probes [PDF]

Genome Research, 1999
We report the development of a self-contained (homogeneous), single-tube assay for the genotyping of single-nucleotide polymorphisms (SNPs), which does not rely on fluorescent oligonucleotide probes. The method, which we call Tm-shift genotyping, combines allele-specific PCR with the discrimination between amplification products by their melting ...
S, Germer, R, Higuchi
openaire   +2 more sources

Analysis of oligonucleotide microarrays by 3′ end labeling using fluorescent nucleotides and terminal transferase

BioTechniques, 2006
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single ...
Cesar E. Guerra
doaj   +1 more source