Results 31 to 40 of about 148,581 (265)

Generation of Amplifiable Genome-Specific Oligonucleotide Probes and Libraries

BioTechniques, 2002
Here we describe a process for the generation of oligonucleotide libraries representative of a given nucleic acid. Starting from a random pool of DNA oligonucleotides, the technique selects only those that hybridize to the nucleic acid template.
Ivan Brukner, Guy A. Tremblay, Bruno Paquin   +2 more
doaj   +1 more source

Oligonucleotide microarray analysis of aminoallyl-labeled cDNA targets from linear RNA amplification

BioTechniques, 2004
Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe ...
Pal Kaposi-Novak, Ju-Seog Lee, Arsen Mikaelyan, Vyomesh Patel, Snorri S. Thorgeirsson   +4 more
doaj   +1 more source

Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization

BioTechniques, 2003
Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products.
V. Abécassis, L. Jaffrelo, D. Rickman, L. Aggerbeck, C. Herbert, G. Truan, D. Pompon   +6 more
doaj   +1 more source

Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide

Cells, 2021
Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Molecular probe technology detects bacteria without culture

BMC Microbiology, 2012
Background Our ultimate goal is to detect the entire human microbiome, in health and in disease, in a single reaction tube, and employing only commercially available reagents.
Hyman Richard W, St Onge Robert P, Kim Hyunsung, Tamaresis John S, Miranda Molly, Aparicio Ana, Fukushima Marilyn, Pourmand Nader, Giudice Linda C, Davis Ronald W   +9 more
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Effect of Secondary Structure on Single Nucleotide Polymorphism Detection with a Porous Microarray Matrix; Implications for Probe Selection

BioTechniques, 2003
Oligonucleotide arrays capable of detecting single nucleotide polymorphisms (SNPs) from amplified nucleic acid have many applications. The expected SNP is usually placed approximately in the center of the probe to ensure the maximum shift in Tm between ...
R.M. Anthony, A.R.J. Schuitema, A.B. Chan, P.J. Boender, P.R. Klatser, L. Oskam   +5 more
doaj   +1 more source

GCSscore: an R package for differential gene expression analysis in Affymetrix/Thermo-Fisher whole transcriptome microarrays

BMC Genomics, 2021
Background Despite the increasing use of RNAseq for transcriptome analysis, microarrays remain a widely-used methodology for genomic studies. The latest generation of Affymetrix/Thermo-Fisher microarrays, the ClariomD/XTA and ClariomS array, provide a ...
Guy M. Harris, Shahroze Abbas, Michael F. Miles   +2 more
doaj   +1 more source

Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer

Molecular Oncology, EarlyView.
Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni, Antonella Palmese, Stefano Scalera, Laura Cipriani, Davide Mascolo, Patrizia Vici, Teresa Arcuri, Lorena Filomeno, Eriseld Krasniqi, Giovanni Blandino, Giulia Bon, Marcello Maugeri‐Saccà   +11 more
wiley   +1 more source