Results 271 to 280 of about 148,528 (314)

Microarray oligonucleotide probes.

Methods in enzymology, 2006
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or ...
David P, Kreil, Roslin R, Russell, Steven, Russell   +2 more
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An oligonucleotide probe specific for Onchocerca volvulus

Molecular and Biochemical Parasitology, 1989
A genomic DNA library of a Liberian strain of Onchocerca volvulus was prepared in the vector bacteriophage lambda gt10. The library was differentially screened by hybridisation with radiolabelled total DNA from the homologous parasite, two heterologous Onchocerca parasites (Onchocerca gibsoni and Onchocerca gutturosa) and human liver cells.
W, Harnett, A E, Chambers, A, Renz, R M, Parkhouse   +3 more
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Dimeric oligonucleotide probes enhance diagnostic macroarray performance

Journal of Microbiological Methods, 2011
Disease management can be improved with rapid and accurate pathogen detection and identification techniques. Here we describe the development of a macroarray diagnostic technique with enhanced detection sensitivity and only small reduction in specificity.
Evans N, Njambere, Bruce B, Clarke, Ning, Zhang   +2 more
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Acridinium ester‐labelled DNA oligonucleotide probes

Journal of Bioluminescence and Chemiluminescence, 1989
AbstractChemiluminescent acridinium ester derivatives have been synthesized and covalently attached to suitably modified synthetic DNA oligonucleotides. Attachment of acridinium ester label to primary aliphatic amine group(s) present in the synthetic DNA probe molecule is rapid and efficient.
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Oligonucleotide probes for DNA fingerprinting in dogs

Journal of Animal Breeding and Genetics, 1994
SummaryTen different oligonucleotide probes were evaluated for DNA fingerprinting in dogs. Seven probes are able to detect polymorphic bands. Probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification. The probabilities that two individuals from different breeds have the same DNA fingerprint pattern are 1.7 × 10−7, 5.5 × 10−8 ...
M, Jung, K, Wilke, H, Geldermann
openaire   +2 more sources

High-Density GeneChip Oligonucleotide Probe Arrays

2002
High-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. Photolithographic DNA synthesis has enabled the large-scale production of GeneChip probe arrays containing hundreds of thousands of oligonucleotide sequences on a glass "chip" about 1.5 cm2 in size. The
Glenn H, McGall, Fred C, Christians
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Oligonucleotide probes for DNA fingerprinting in horses

Journal of Animal Breeding and Genetics, 1993
Summary10 different oligonucleotide probes were evaluated for DNA fingerprinting in horses. Five probes were able to detect polymorphic bands. The probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification and were used to analyze a population of Hannoveranian horses.
K, Wilke, M, Weimann, M, Jung, H, Geldermann   +3 more
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DNA fingerprinting in cattle using oligonucleotide probes

Animal Genetics, 1991
Summary. Oligonucleotide probes specific for simple tandem repeat sequences produce individual specific DNA fingerpints in man and all animal species tested so far. Here 11 different synthetic probes were hybridized to bovine genomic DNAs which had been digested with the restriction endonucleases Hinfl, Alu I and Hae III.
J, Buitkamp, H, Zischler, J T, Epplen, H, Geldermann   +3 more
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HLA class II typing using oligonucleotide probes

Genetic Epidemiology, 1989
AbstractA DQβ locus‐specific oligonucleotide probe is used to identify DQβ alleles present in six insulin‐dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer.
D M, Robinson, S, Holbeck, C, Seyfried, P, Byers, J, Palmer, G T, Nepom   +5 more
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Oligonucleotide probes for bacterial acylcarrier protein genes

Biochimie, 1985
Using a recently-introduced rapid manual method, we have synthesized a family of thirty six individual oligonucleotides of unique sequence (18-mers), which correspond to the conserved amino acid sequence, GADSLD, found at the 4'-phosphopantetheine-binding site of the acylcarrier component of bacterial and plant fatty acid synthases.
R S, Hale, P F, Leadlay
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