Results 251 to 260 of about 222,321 (300)

Using Synthetic Oligonucleotides as Probes

Current Protocols in Molecular Biology, 1988
AbstractThe protocols in this unit describe procedures for using mixtures of 32P‐labeled oligonucleotides to screen recombinant DNA clones bound to nitrocellulose filters. A partial amino acid sequence of a protein is used to predict the nucleotide sequence of the gene that would encode it.
A, Duby, K A, Jacobs, A, Celeste
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Triplex Tethered Oligonucleotide Probes

Journal of the American Chemical Society, 1996
RNA molecules are believed to possess highly complex structures containing singleand double-stranded regions as well as loops, bulges, and pseudoknots.1 This structural complexity severely complicates efforts to design structureand/or sequenceselective RNA-binding molecules.2,3 Recently we described a family of molecules for the sequenceand structure ...
Arikha C. Moses, Alanna Schepartz
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Oligonucleotide probe design — a new approach

Nature, 1994
The use of oligonucleotides for gene amplification, as diagnostic probes or antisense-based drugs can be optimized by minimizing the possibility of nonspecific hybridization.
M, Mitsuhashi, A, Cooper, M, Ogura, T, Shinagawa, K, Yano, T, Hosokawa   +5 more
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Probe Design Strategies for Oligonucleotide Microarrays

2016
Oligonucleotide microarrays have been widely used for gene detection and/or quantification of gene expression in various samples ranging from a single organism to a complex microbial assemblage. The success of a microarray experiment, however, strongly relies on the quality of designed probes.
Parisot, Nicolas, Peyretaillade, Eric, Dugat-Bony, Eric, Denonfoux, Jérémie, Mahul, Antoine, Peyret, Pierre   +5 more
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Optimized oligonucleotide probes for DNA fingerprinting

ELECTROPHORESIS, 1988
AbstractThe three different simple repetitive oligonucleotide probes (CT)8, (CAC)5 and (TCC)5 were hybridized to a panel of human DNAs which had been digested with the restriction endonucleases Alu I, Hinf I and Mbo I. The resulting DNA fingerprints were analyzed and different parameters calculated, such as the maximal mean allele frequency and the ...
R, Schäfer, H, Zischler, U, Birsner, A, Becker, J T, Epplen   +4 more
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Oligonucleotide probes functionalization of nanogap electrodes

ELECTROPHORESIS, 2017
AbstractNanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current‐voltage measurements. Nevertheless, application of standard current‐voltages measurements used to
Rosa Letizia Zaffino, Mònica Mir, Josep Samitier   +2 more
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In situ hybridization with oligonucleotide probes

2002
Publisher Summary In situ hybridization (ISH) is an important method for tracing the regional and cellular sites of gene expression (mRNA distribution) within a tissue. This method comes into its own when applied to the intermingled cell types of the brain.
W, Wisden, B J, Morris
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Microarray oligonucleotide probes.

Methods in enzymology, 2006
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or ...
David P, Kreil, Roslin R, Russell, Steven, Russell   +2 more
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An oligonucleotide probe specific for Onchocerca volvulus

Molecular and Biochemical Parasitology, 1989
A genomic DNA library of a Liberian strain of Onchocerca volvulus was prepared in the vector bacteriophage lambda gt10. The library was differentially screened by hybridisation with radiolabelled total DNA from the homologous parasite, two heterologous Onchocerca parasites (Onchocerca gibsoni and Onchocerca gutturosa) and human liver cells.
W, Harnett, A E, Chambers, A, Renz, R M, Parkhouse   +3 more
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Dimeric oligonucleotide probes enhance diagnostic macroarray performance

Journal of Microbiological Methods, 2011
Disease management can be improved with rapid and accurate pathogen detection and identification techniques. Here we describe the development of a macroarray diagnostic technique with enhanced detection sensitivity and only small reduction in specificity.
Evans N, Njambere, Bruce B, Clarke, Ning, Zhang   +2 more
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