Results 71 to 80 of about 1,424,575 (357)

Probe selection for high-density oligonucleotide arrays [PDF]

Proceedings of the National Academy of Sciences, 2003
High-density oligonucleotide microarrays enable simultaneous monitoring of expression levels of tens of thousands of transcripts. For accurate detection and quantitation of transcripts in the presence of cellular mRNA, it is essential to design microarrays whose oligonucleotide probes produce hybridization intensities that accurately reflect the ...
Paul L. Kaplan, Mei-Mei Shen, Teresa Webster, Joy Fang, Stefan Bekiranov, Rui Mei, David Kulp, Mike Mittmann, Thomas B. Ryder, Wei-Min Liu, Fred Christians, Gang Lu, Earl Hubbell   +12 more
openaire   +3 more sources

Activation of NF‐κB Signaling by Optogenetic Clustering of IKKα and β

Advanced Biology, EarlyView.
This study presents an optogenetic approach for graded clustering of eGFP‐fused proteins using an eGFP‐specific nanobody and engineered Cryptochrome 2 variants. The method enables potent, reversible activation of NF‐κB signaling via endogenous pathways, as confirmed by RNA sequencing. This versatile system provides a spatially and temporally controlled
Alexandra Anna Maria Fischer, Markus Michael Kramer, Miguel Baños, Merlin Moritz Grimm, Manfred Fliegauf, Bodo Grimbacher, Gerald Radziwill, Sven Rahmann, Wilfried Weber   +8 more
wiley   +1 more source

A comparison of alternative 60-mer probe designs in an in-situ synthesized oligonucleotide microarray

BMC Genomics, 2006
Background DNA microarrays have proven powerful for functional genomics studies. Several technologies exist for the generation of whole-genome arrays.
Fairbanks Benjamin D, Hume Patrick S, Karimpour-Fard Anis, Leiske Danielle L, Gill Ryan T   +4 more
doaj   +1 more source

Identification of Chromosomes and Chromosome Rearrangements in Crop Brassicas and Raphanus sativus: A Cytogenetic Toolkit Using Synthesized Massive Oligonucleotide Libraries

Frontiers in Plant Science, 2020
Crop brassicas include three diploid [Brassica rapa (AA; 2n = 2x = 16), B. nigra (BB; 2n = 2x = 18), and B. oleracea (CC; 2n = 2x = 20)] and three derived allotetraploid species.
Neha Agrawal, Neha Agrawal, Mehak Gupta, Surinder S. Banga, JS (Pat) Heslop-Harrison, JS (Pat) Heslop-Harrison   +5 more
doaj   +1 more source

The biophysics of DNA hybridization with immobilized oligonucleotide probes

Biophysical Journal, 1995
A mathematical model based on receptor-ligand interactions at a cell surface has been modified and further developed to represent heterogeneous DNA-DNA hybridization on a solid surface. The immobilized DNA molecules with known sequences are called probes, and the DNA molecules in solution with unknown sequences are called targets in this model. Capture
Steven E. McKenzie, David J. Graves, Vincent Chan   +2 more
openaire   +3 more sources

Evaluating oligonucleotide properties for DNA microarray probe design [PDF]

Nucleic Acids Research, 2010
Most current microarray oligonucleotide probe design strategies are based on probe design factors (PDFs), which include probe hybridization free energy (PHFE), probe minimum folding energy (PMFE), dimer score, hairpin score, homology score and complexity score.
Xia, Xiao-Qin, Jia, Zhenyu, Porwollik, Steffen, Long, Fred, Hoemme, Claudia, Ye, Kai, Muller-Tidow, Carsten, McClelland, Michael, Wang, Yipeng   +8 more
openaire   +5 more sources

Mixed‐Dimensional 0D‐DNA‐2D Heterostructures Beyond van der Waals: A DNA‐Templated Strategy for Optoelectronic Tunability

Advanced Functional Materials, EarlyView.
A two‐step DNA metallization process is presented for the modular assembly of metal sulfide nanoparticles (NPs) on MoS2, with nanoscale control over their separation and ability to concomitantly assemble different kind of NPs. This allowed to tailor the photoinduced electrical response of phototransistors to different wavelengths, according to the ...
Kai Chen, Haosen Miao, Stoichko Dimitrov, Matteo Palma   +3 more
wiley   +1 more source

Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging

Communications Biology, 2021
Tomov et al. utilize DNA-PRISM to allow for multiplexed imaging of cultured cells using antibodies modified with oligonucleotide probes. The differentiation of iPSCs to cortical and motor neurons is characterized in model cultures, relevant for use in ...
Martin L. Tomov, Alison O’Neil, Hamdah S. Abbasi, Beth A. Cimini, Anne E. Carpenter, Lee L. Rubin, Mark Bathe   +6 more
doaj   +1 more source

Facile SNP detection using bifunctional, cross-linking oligonucleotide probes

Nucleic Acids Research, 2008
A facile, sensitive method for detecting specific sequences of oligonucleotides was developed. Detection of DNA sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking ...
Xiaohua Peng, M. Greenberg
semanticscholar   +1 more source

A Sustainable Biotechnology Approach for Mineral Separation

Advanced Functional Materials, EarlyView.
Silver‑binding peptides are first identified through phage‐display screening, and then engineered into multifunctional and responsive peptides or proteins. These tailored biomolecules can selectively capture and aggregate silver particles or ions from complex mixtures, enabling rapid and efficient silver separation and recovery.
Guangze Yang, Yun Liu, Shankar Raj Devkota, Yue Hui, Ruilin Zhao, Yang Li, David A. Weitz, Chun‐Xia Zhao   +7 more
wiley   +1 more source