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Antisense oligonucleotides in cutaneous therapy
Pharmacology & Therapeutics, 2001Antisense oligonucleotides have been the subject of intense interest as research tools to elucidate the functions of gene products and as therapeutic agents. Initially, their mode of action was poorly understood and the biological effects of oligonucleotides were often misinterpreted.
Paul J. White, Christopher J. Wraight
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History of Antisense Oligonucleotides
2003Biological science is a rapidly flowing experimental stream, at times encountering a dam that impedes further progress. At such a pomt, a single crack may induce a major breakthrough Discovery of the double helical structure of DNA in 1953 (1) caused such an event, with flooding of new information into the area now known as molecular biology.
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Antisense Oligonucleotides: Promise and Reality
Annual Review of Pharmacology and Toxicology, 2001Antisense oligonucleotides have been used for more than a decade to downregulate gene expression. Phosphodiester oligonucleotides are nuclease sensitive, and the more nuclease-resistant phosphorothioate oligonucleotides are now in common use in the laboratory and have entered clinical trials.
Cy A. Stein, Irina V. Lebedeva
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Mini-antisense Oligonucleotides
Nucleosides and Nucleotides, 1997Abstract A new strategy of selective DNA target modification was proposed. The using of reactive derivatives of short oligonucleotides in the presence of flanking effector pair allows one to modify DNA target only when the perfect complementary complex of DNA target and oligonucleotide tandem is formed.
Dmitrii V. Pyshnyi +4 more
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Antisense Oligonucleotide Therapy in Urology
Journal of Urology, 2002Antisense oligonucleotides are short modified DNA or RNA molecules designed to bind selectively messenger RNA and inhibit synthesis of the encoded protein. In the last 20 years antisense technology has emerged as an exciting and promising strategy, especially for treating cancer.
Ingo Kausch, A. Böhle
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Progress in Antisense Oligonucleotide Therapeutics
Annual Review of Pharmacology and Toxicology, 1996The past several years have seen substantial progress in the development of antisense oligonucleotides as pharmacological tools and as therapeutic agents. With properly designed and executed experiments, it has been possible to demonstrate selective inhibition of gene expression, owing to an antisense mechanisms of action both in cell culture-based ...
Stanley T. Crooke, C F Bennett
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Specificity of antisense oligonucleotides
Perspectives in Drug Discovery and Design, 1996Antisense oligodeoxynucleotides that are sufficiently long to specify unique species of mRNA may direct ribonuclease H (RNase H) to cleave nontargeted mRNAs at sites of partial complementarity, both in cell-free systems and in living cells. Specificity of antisense action against selected gene expression may be achieved by increasing the stringency of ...
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Antisense Oligonucleotides as Research Tools
2003The use of antisense oligonucleotides as both research tools and therapeutic molecules has emerged as a powerful alternative to small molecule inhibitors. Antisense oligonucleotides are short pieces of chemically modified DNA designed to hybridize to specific mRNA sequences present in the target gene.
Nicholas M. Dean +2 more
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Antisense oligonucleotide strategies in physiology
Molecular and Cellular Endocrinology, 1994Antisense oligonucleotides can inhibit gene expression in living cells by binding to complementary sequences of DNA, RNA or mRNA. The mechanisms include inhibition of RNA synthesis, RNA splicing, mRNA export, binding of initiation factors, assembly of ribosome subunits and of sliding of the ribosome along the mRNA coding sequence.
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Antisense Oligonucleotides: The State of the Art
Current Medicinal Chemistry, 2005The use of antisense oligonucleotides as therapeutic agents has generated considerable enthusiasm in the research and medical community. Antisense oligonucleotides as therapeutic agents were proposed as far back as in the 1970s when the antisense strategy was initially developed.
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