Results 271 to 280 of about 584,539 (379)

Interplay of Structure, Hydration and Thermal Stability in Formacetal Modified Oligonucleotides: RNA May Tolerate Nonionic Modifications Better than DNA

, 2009
Andrej Kolarovič, Emma Schweizer, Emily M. Greene, Mark Gironda, Pradeep S. Pallan, Martin Egli, Eriks Rozners   +6 more
openalex   +1 more source

Genome‐Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle‐Mediated siRNA Delivery

Advanced Science, EarlyView.
A genome‐wide CRISPR screen and identifies PIK3CA as a key regulator of lipid nanoparticle (LNP) uptake is performed. Modulating PIK3CA with small‐molecule inhibitor BAY1082439 enhanced LNP uptake, siRNA delivery, and gene silencing in a variety of epithelial cells, and improved therapeutic outcomes in vivo.
Wenhan Wang, Kangfu Chen, Zongjie Wang
wiley   +1 more source

The role of exosomes as oligonucleotide delivery system for managing α-synuclein in Parkinson's disease: A systematic review of <i>in vivo</i> studies. [PDF]

Mol Ther Nucleic Acids
Salarpour S, Dourandish F, Salajegheh A, Hashemi S.   +3 more
europepmc   +1 more source

Designing chemically modified oligonucleotides for targeted gene silencing.

Chemistry and Biology, 2012
G. Deleavey, M. Damha
semanticscholar   +1 more source

MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection

Advanced Science, EarlyView.
MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani, Luca Capelli, Denise Di Lena, Alejandro Chamorro‐Garcia, Rudy Ippodrino, Alessandro Porchetta, Alessandro Bertucci   +6 more
wiley   +1 more source

High-Throughput Single-Molecule Microscopy with Adaptable Spatial Resolution Using Exchangeable Oligonucleotide Labels [PDF]

Klarinda de Zwaan, Ran Huo, Myron N.F. Hensgens, Hannah Lena Wienecke, Miyase Tekpınar, Hylkje Geertsema, Kristin S. Grußmayer   +6 more
openalex   +1 more source

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells

Advanced Science, EarlyView.
A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Mengru Lin, Jiale Sun, Yuqing Mao, Yuhao Tang, Fuan Wang, Zhihong Liu, Jing Wang   +6 more
wiley   +1 more source

Presence of phosphodiester backbone, but not nucleobases, in the guide's 3' terminal region is necessary for RISC loading and target cleavage in vitro and in vivo. [PDF]

Nucleic Acids Res
O'Reilly D, Furgal RC, Ross VM, Luu E, Gross KY, Hariharan V, Summers A, Cooper D, Allen S, Dahlke C, Rachid MO, Chan IL, Fakih HH, Alterman JF, Echeverria D, Watts JK, Khvorova A.   +16 more
europepmc   +1 more source

Oligonucleotide sequences of primers utilized for real-time qRT-PCR.

, 2015
Ali Hamad, K. Al-Yatama Majda, Abu-Farha Mohamed, Kazem Behbehani, Al Madhoun Ashraf   +4 more
openalex   +2 more sources