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Chitin in egg shells of Onchocerca gibsoni and Onchocerca volvulus
Molecular and Biochemical Parasitology, 1987Chemical analysis of adult females of Onchocerca gibsoni gave estimated chitin contents of 200-500 micrograms (g dry weight)-1. Egg shells from both O. gibsoni and Onchocerca volvulus stained with Calcofluor white and with fluorescent wheat germ agglutinin as shown by fluorescent light microscopy, and bound gold-labelled wheat germ agglutinin as shown ...
Lesley J. Brydon +3 more
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The γ-glutamylcysteine synthetase of Onchocerca volvulus
Molecular and Biochemical Parasitology, 2000The tripeptide glutathione (GSH) plays an important role in the maintenance of the intracellular thiol redox state and in detoxification processes. The intracellular GSH level depends on glutathione reductase as well as on GSH synthesis. The first and rate limiting step in the synthetic pathway is catalysed by gamma-glutamylcysteine synthetase (gamma ...
Eva Liebau +4 more
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Immunohistological studies on Onchocerca volvulus paramyosin
Parasitology Research, 2009Paramyosin is a muscular protein exclusively found in invertebrate species, which has been proposed as a vaccine candidate against infections with Schistosoma mansoni and Brugia malayi. Here, we report the studies on the distribution of Onchocerca volvulus paramyosin, designated OvPmy, in different O.
Klaus D. Erttmann, Dietrich W. Büttner
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Morphology ofOnchocerca volvulus
American Journal of Clinical Pathology, 1972Onchocercal nodules from 18 Africans were studied. Each nodule contained adult worms of Onchocerca volvulus . Male and female worms were reconstructed from serial paraffin sections, and the morphologic features of the adult worms and microfilariae described.
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An oligonucleotide probe specific for Onchocerca volvulus
Molecular and Biochemical Parasitology, 1989A genomic DNA library of a Liberian strain of Onchocerca volvulus was prepared in the vector bacteriophage lambda gt10. The library was differentially screened by hybridisation with radiolabelled total DNA from the homologous parasite, two heterologous Onchocerca parasites (Onchocerca gibsoni and Onchocerca gutturosa) and human liver cells.
William Harnett +3 more
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The burden of Onchocerca volvulus in Sudan
Annals of Tropical Medicine & Parasitology, 1998Onchocerciasis has been reported in Sudan since 1908 and now prevails in three endemic regions known as the southern, northern and eastern foci. The southern focus is the largest, with nodule and blindness rates exceeding 80% and 12%, respectively, in certain villages.
Moawia M. Mukhtar +3 more
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Onchocerca volvulus, Wolbachia and River Blindness
2007Chronic infection with filarial nematodes results in development of a suppressive response to an immense parasite burden, thereby limiting pathological and clinical manifestations. However, pro-inflammatory responses to dead and degenerating Onchocerca volvulus worms and release of endosymbiotic Wolbachia bacteria result in corneal opacification ...
Illona Gillette-Ferguson, Eric Pearlman
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Characterization of an Onchocerca-Specific DNA Clone from Onchocerca Volvulus
The American Society of Tropical Medicine and Hygiene, 1987A genomic library of a savanna isolate of Onchocerca volvulus was screened to detect recombinant plasm ids containing highly repeated DNA sequences of this parasite. Four recombinant plasmids were identified which hybridized specifically to Onchocerca DNA, but not to DNA from humans, black flies, Brugia malayi, B. pahangi, or Wuchereria bancrofti.
Dyann F. Wirth +3 more
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Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use
Molecular and Biochemical Parasitology, 1986Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique using sera from individuals infected ...
R. M. E. Parkhouse, Zully Cabrera
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In Vitro Cultivation and Development of Onchocerca volvulus and Onchocerca lienalis Microfilariae
The American Journal of Tropical Medicine and Hygiene, 1996Onchocerca volvulus and O. lienalis skin-derived microfilariae (mf) were cultured in vitro; parasite viability was assessed at intervals by measuring their ability to develop in Simulium ornatum. In the presence of monkey kidney feeder cells, both species retained full viability when cultured for up to 5 hr before intrathoracic injection into Simulium.
Senyo Tagboto, Simon Townson
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