Results 51 to 60 of about 48,989 (163)

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

mBio
We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion ...
Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin   +5 more
doaj   +1 more source

The Rhizobium etli trpB Gene Is Essential for an Effective Symbiotic Interaction with Phaseolus vulgaris

Molecular Plant-Microbe Interactions, 1999
A mutant strain (CTNUX4) of Rhizobium etli carrying Tn5 unable to grow with ammonium as the sole nitrogen source was isolated and characterized. Sequence analysis showed that Tn5 is inserted into a trpB (tryptophan synthase)-homologous gene.
Rosarita Taté, Anna Riccio, Emilia Caputo, Michele Cermola, Renee Favre, Eduardo J. Patriarca   +5 more
doaj   +1 more source

Horizontal gene transfer and diverse functional constrains within a common replication-partitioning system in Alphaproteobacteria: the repABC operon

BMC Genomics, 2009
Background The repABC plasmid family, which is extensively present within Alphaproteobacteria, and some secondary chromosomes of the Rhizobiales have the particular feature that all the elements involved in replication and partitioning reside within one ...
Cevallos Miguel A, González Víctor, Vázquez-Castellanos Jorge F, Castillo-Ramírez Santiago   +3 more
doaj   +1 more source

Operon and non-operon gene clusters in the C. elegans genome [PDF]

WormBook, 2014
Nearly 15% of the ~20,000 C. elegans genes are contained in operons, multigene clusters controlled by a single promoter. The vast majority of these are of a type where the genes in the cluster are ~100 bp apart and the pre-mRNA is processed by 3' end formation accompanied by trans-splicing.
Thomas, Blumenthal, Paul, Davis, Alfonso, Garrido-Lecca   +2 more
openaire   +2 more sources

Relatively slow stochastic gene-state switching in the presence of positive feedback significantly broadens the region of bimodality through stabilizing the uninduced phenotypic state. [PDF]

PLoS Computational Biology, 2018
Within an isogenic population, even in the same extracellular environment, individual cells can exhibit various phenotypic states. The exact role of stochastic gene-state switching regulating the transition among these phenotypic states in a single cell ...
Hao Ge, Pingping Wu, Hong Qian, Xiaoliang Sunney Xie   +3 more
doaj   +1 more source

RegulonDB (version 5.0): Escherichia coli K-12 transcriptional regulatory network, operon organization, and growth conditions

Nucleic Acids Res., 2005
RegulonDB is the internationally recognized reference database of Escherichia coli K-12 offering curated knowledge of the regulatory network and operon organization. It is currently the largest electronically-encoded database of the regulatory network of
Heladia Salgado, Socorro Gama-Castro, Martín Peralta-Gil, Edgar Díaz-Peredo, Fabiola Sánchez-Solano, Alberto Santos-Zavaleta, Irma Martínez-Flores, V. Jímenez-Jacinto, César Bonavides-Martínez, J. Segura-Salazar, A. Martínez-Antonio, J. Collado-Vides   +11 more
semanticscholar   +1 more source

Tracing the ancestry of operons in bacteria [PDF]

Bioinformatics, 2017
Abstract Motivation Complexity is a fundamental attribute of life. Complex systems are made of parts that together perform functions that a single component, or subsets of components, cannot. Examples of complex molecular systems include protein structures such as the F1Fo-ATPase, the ribosome,
Huy N. Nguyen, Ashish Jain, Oliver Eulenstein, Iddo Friedberg   +3 more
openaire   +2 more sources

Mutations in SilS and CusS/OmpC represent different routes to achieve high level silver ion tolerance in Klebsiella pneumoniae

BMC Microbiology, 2022
Background Silver ions have potent broad-spectrum antimicrobial activity and are widely incorporated into a variety of products to limit bacterial growth.
Charlotte A. Woolley, J. Mark Sutton, Matthew E. Wand   +2 more
doaj   +1 more source

Modeling operon dynamics: the tryptophan and lactose operons as paradigms

Comptes Rendus. Biologies, 2004
Understanding the regulation of gene control networks and their ensuing dynamics will be a critical component in the understanding of the mountain of genomic data being currently collected. This paper reviews recent mathematical modeling work on the tryptophan and lactose operons which are, respectively, the classical paradigms for repressible and ...
Yildirim, N, Mackey, MC, Santillan, M
openaire   +3 more sources

Using origami and Shrinky Dinks to create active learning activities to tackle two microbiology concepts: cell structure differences and operon regulation

Journal of Microbiology & Biology Education
This paper presents two low-cost hands-on activities designed to enhance student understanding and address the pedagogical challenges faced by microbiology professors in teaching concepts related to cell structure and gene regulation.
Manuela Tripepi, Hannah M. Schapiro
doaj   +1 more source