Results 91 to 100 of about 445,901 (262)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Horn-Schunck Optical Flow with a Multi-Scale Strategy
Image Processing On Line, 2013 The seminal work of Horn and Schunck is the first variational method for optical flow estimation. It introduced a novel framework where the optical flow is computed as the solution of a minimization problem.
Enric Meinhardt-Llopis +2 more
doaj
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Research on traditional and deep learning strategies based on optical flow estimation - a review
Journal of King Saud University: Computer and Information SciencesOptical flow estimation captures the motion information of objects in a scene through analyzing the displacement of pixels in an image over time. This technology provides a powerful tool for vision systems, allowing them to understand and perceive ...
Yifan Wang +8 more
doaj +1 more source
Discrete Optimization for Optical Flow
, 2015 We propose to look at large-displacement optical flow from a discrete point of view. Motivated by the observation that sub-pixel accuracy is easily obtained given pixel-accurate optical flow, we conjecture that computing the integral part is the hardest piece of the problem.
Moritz Menze +2 more
openaire +2 more sources
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
BuildingsSoil particle size distribution is a critical parameter in geotechnical and hydraulic engineering, particularly in applications such as dam seepage monitoring, building foundation assessments, and sediment transport. This study presents a novel algorithm
Shuangping Li +6 more
doaj +1 more source