Results 251 to 260 of about 99,235 (279)

Optical PCR: Genomic analysis by long-range PCR and optical mapping

Mammalian Genome, 1999
Optical mapping is an approach for the rapid, automated, non-electrophoretic construction of ordered restriction maps of DNA from ensembles of single molecules. Previously, we used optical mapping to make high-resolution maps of large insert clones such as bacterial artificial chromosomes (BAC) and large genomic DNA molecules.
J, Skiadas, C, Aston, A, Samad, T S, Anantharaman, B, Mishra, D C, Schwartz   +5 more
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A shotgun optical map of the entire Plasmodium falciparum genome

Nature Genetics, 1999
The unicellular parasite Plasmodium falciparum is the cause of human malaria, resulting in 1.7-2.5 million deaths each year. To develop new means to treat or prevent malaria, the Malaria Genome Consortium was formed to sequence and annotate the entire 24.6-Mb genome.
Z, Lai, J, Jing, C, Aston, V, Clarke, J, Apodaca, E T, Dimalanta, D J, Carucci, M J, Gardner, B, Mishra, T S, Anantharaman, S, Paxia, S L, Hoffman, J, Craig Venter, E J, Huff, D C, Schwartz   +14 more
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Identification and Characterization of Chromothripsis by Optical Genome Mapping

Optical genome mapping (OGM) is a robust method for identifying and characterizing chromothripsis. While conventional short-read sequencing techniques encounter challenges in detecting chromothripsis due to limitations in read length and difficulties in analyzing repetitive sequences, OGM leverages the visualization of long DNA molecules to overcome ...
Pascal, Chambon, Mathilde, Quibeuf, Anne Marie, Guerrot   +2 more
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Genomics via optical mapping. III: Contiging genomic DNA.

Proceedings. International Conference on Intelligent Systems for Molecular Biology, 2000
In this paper, we describe our algorithmic approach to constructing an alignment of (contiging) a set of restriction maps created from the images of individual genomic (uncloned) DNA molecules digested by restriction enzymes. Generally, these DNA segments are sized in the range of 1-4 Mb.
Thomas S. Anantharaman, Bud Mishra, David C. Schwartz   +2 more
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Cytogenetics and the Revolution of Optical Genome Mapping in the Diagnosis of Diseases

Discovery Medicine
The study of chromosomal shape, characteristics, and behavior in somatic cell division (mitosis) during growth and development and in germ cell division (meiosis) during reproduction is known as cytogenetics. Many techniques can be used for cytogenetics, including fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), multicolor FISH (M ...
Osama M, Al-Amer, Yahya, Khubrani
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Mapping the genome one molecule at a time — optical mapping

Nature, 1995
Akhtar H. Samad, Wei Wen Cai, Xinghua Hu, Benjamin Irvin, Junping Jing, Jason Reed, Xun Meng, John Huang, Edward Huff, Brett Porter, Alex Shenkar, Thomas Anantharaman, Bhubaneswar Mishra, Virginia Clarke, Eileen Dimalanta, Joanne Edington, Catharina Hiort, Roderic Rabbah, John Skiada, David C. Schwartz   +19 more
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Optical Genome Mapping: A Machine-Based Platform in Cytogenomics

Optical genome mapping (OGM) has generated excitement following decades of research and development. Now, commercially available technical platforms have been used to compare various other cytogenetic and cytogenomic technologies, including karyotype, microarrays, and DNA sequencing, with impressive results.
Jing Christine, Ye, Guilin, Tang
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Genomic Mapping of Kjer Dominant Optic Atrophy

Archives of Ophthalmology, 1997
Kjer DOMINANToptic atrophy (DOA) is a rare primary degeneration of the retinal ganglion cell. In this issue of theArchives, 2 articles describing the genetic analysis of single, large, affected families confirm the initial genomic mapping of this disease to chromosome 3q27-3q28 and refine the localization of the gene to a 4 to 7 centimorgan region.1 ...
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Deciphering Genomic Complexity of Multiple Myeloma Using Optimized Optical Genome Mapping

The Journal of Molecular Diagnostics
The genomic evaluation of multiple myeloma in routine diagnostics involves isolating plasma cells expressing CD138, usually followed by fluorescence in situ hybridization analyses. However, cell sorting often yields a limited number of cells, restricting the number of probes that can be used and limiting the analysis to a few markers required for ...
Hélène Guermouche, Pauline Roynard, Francesca Servoli, Valentin Lestringant, Benoît Quilichini, Christine Terré, Sabine Defasque, Catherine Roche-Lestienne, Dominique Penther, Agnès Daudignon   +9 more
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Genomic mapping: a statistical and algorithmic analysis of the optical mapping system

2015
The ability to conduct whole genome analysis of variation is slowly becoming a reality with both improvements in biotechnology and advancements in data analysis. However, large-scale de novo sequencing still remains a formidable task for complex plant and mammalian genomes.
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