Results 71 to 80 of about 469,303 (219)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Design and analysis of a dual-rotation switching mirror mechanism in an off-axis catadioptric zoom optical system

Advances in Mechanical Engineering
A novel dual-rotation switching mirror mechanism is proposed in this paper for field-of-view switching of off-axis catadioptric zoom optical systems applied to airborne electro-optical payloads.
Songnian Tan, Yongsen Xu, YeFei Wang, Yuan Yao, Zhichong Wang, Lei Shi   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Pixel-super-resolved lens-free quantitative phase microscopy with partially coherent illumination

npj Nanophotonics
Lens-free on-chip microscopy (LFOCM) has been widely utilized in digital pathology, drug screening, point-of-care testing (POCT), and quantitative phase imaging (QPI) due to its high throughput imaging capability and compactness.
Yang Chen, Xuejuan Wu, Linpeng Lu, Jiasong Sun, Runnan Zhang, Wenhui Lin, Yufan Chen, Maciej Trusiak, Peng Gao, Chao Zuo   +9 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Deep-learning-enabled temporally super-resolved multiplexed fringe projection profilometry: high-speed kHz 3D imaging with low-speed camera

PhotoniX
Recent advances in imaging sensors and digital light projection technology have facilitated rapid progress in 3D optical sensing, enabling 3D surfaces of complex-shaped objects to be captured with high resolution and accuracy.
Wenwu Chen, Shijie Feng, Wei Yin, Yixuan Li, Jiaming Qian, Qian Chen, Chao Zuo   +6 more
doaj   +1 more source

Unmanned Helicopter Airborne Fourier Transform Infrared Spectrometer Remote Sensing System for Hazardous Vapors Detection

Applied Sciences
The rapid development of unmanned aerial vehicles (UAVs) provides a new application mode for gas remote sensing. Compared with fixed observation and vehicle-mounted platforms, a Fourier transform infrared spectrometer (FTIR) integrated in the UAV can ...
Zhengyang Shi, Min Huang, Lulu Qian, Wei Han, Guifeng Zhang, Xiangning Lu   +5 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Aberration-corrected differential phase contrast microscopy with annular illuminations

Opto-Electronic Science
Quantitative phase imaging (QPI) enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes, revolutionizing label-free microscopy in cellular research.
Yao Fan, Chenyue Zheng, Yefeng Shu, Qingyang Fu, Lixiang Xiong, Guifeng Lu, Jiasong Sun, Chao Zuo, Qian Chen   +8 more
doaj   +1 more source