Results 101 to 110 of about 661,323 (302)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Research study of a fundus tracker for experiments in stabilized vision [PDF]

Design and operating characteristics of electro-optical instrument for tracking of retinal blood vessels in back of ...
Crane, H. D., Kelly, D. H.
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

The CCD and readout electronics for the OMC instrument on Integral [PDF]

, 2003
The Optical Monitoring Camera (OMC) on ESA's Integral gamma-ray astronomy satellite is devoted to optical wavelength observations simultaneously covering the same field-of-view as the gamma-ray and X-ray instruments.
Bradley, LJ, Culhane, JL, Dibbens, AP, Jordan, B, Smith, A, Thomas, PD, Walton, DM   +6 more
core  

Microscale application of column theory for high resolution force and displacement sensing

, 2004
We present the design, fabrication and experimental validation of a novel device that exploits the amplification of displacement and attenuation of structural stiffness in the post-buckling deformation of slender columns to obtain pico-Newton force and ...
A. V. Desai, B. A. Samuel, Hartman A. B., M. A. Haque   +3 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Measurement Research of Free-form Lens Optical Parameters Based on Transmission Fringe Deflectometry

IEEE Photonics Journal
This paper presents a measurement system based on the transmission fringe deflectometry, which is designed to facilitate the rapid attainment of the free-form lens optical parameters, including spherical power, cylindrical power, and prismatic power.
Xiaoxiao Wei, Xiaobing He, Zhenqiu Dai, Hao Zhang   +3 more
doaj   +1 more source

An Optical Altitude Indicator for Night Landing [PDF]

One of the most ingenious of the devices intended for use in night landing, especially emergency landing, is a very simple optical instrument known as the Jenkins night altitude indicator. The design and operation of this instrument, which allows a pilot
Warner, John A C
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source