Results 111 to 120 of about 1,945,269 (285)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Lanthanide molecular cluster-aggregates as the next generation of optical materials. [PDF]

Chem Sci, 2023
Gálico DA, Santos Calado CM, Murugesu M.   +2 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

High-order resonance enhancing the mass sensitivity of diamond cantilevers

APL Materials
Diamond has been demonstrated as an exceptional semiconductor for microelectromechanical system (MEMS) sensors, offering high sensitivity and reliability due to its ultra-wide bandgap energy, superior mechanical properties, and high thermal conductivity.
Wen Zhao, Guo Chen, Keyun Gu, Masaya Toda, Yasuo Koide, Meiyong Liao   +5 more
doaj   +1 more source

Alkali metals doped cycloparaphenylene nanohoops: Promising nonlinear optical materials with enhanced performance. [PDF]

Heliyon, 2023
Rasul R, Mahmood T, Ayub K, Joya KS, Anwar F, Saari N, Nawaz R, Gilani MA.   +7 more
europepmc   +1 more source

Reconfigurable photonic metamaterials drive by Coulomb, Lorentz and optical forces

, 2013
Metamaterials offer a huge range of enhanced and novel functionalities that natural materials cannot provide. They promise applications in superresolution imaging, optical data storage, optical filters, polarization control, cloaking, fraud prevention ...
Ou, J-Y., Plum, E., Valente, J., Zheludev, N.I.   +3 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Optical Materials

, 2017
Nikolay L. Kazanskiy, Vsevolod A. Kolpakov   +1 more
  +5 more sources

A Cation-Driven Approach toward Deep-Ultraviolet Nonlinear Optical Materials. [PDF]

Research (Wash D C), 2023
Hu C, Cheng M, Jin W, Han J, Yang Z, Pan S.   +5 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source