Results 51 to 60 of about 247,136 (257)

In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy

Communications Biology, 2023
iCLiPSpy is a simple assay to identify residues important for substrate binding and activity of intramembrane-cleaving proteases (I-CLiPs) in vivo and can be adapted to a screening assay for I-CLiP inhibitor or activator discovery.
Thomas Kupke, Rabea M. Götz, Florian M. Richter, Rainer Beck, Fabio Lolicato, Walter Nickel, Carsten Hopf, Britta Brügger   +7 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Feasibility Study for a Chemical Process Particle Size Characterization System for Explosive Environments Using Low Laser Power

Micromachines, 2020
The industrial particle sensor market lacks simple, easy to use, low cost yet robust, safe and fast response solutions. Towards development of such a sensor, for in-line use in micro channels under continuous flow conditions, this work introduces static ...
Jesse Ross-Jones, Tobias Teumer, Susann Wunsch, Lukas Petri, Hermann Nirschl, Mathias J. Krause, Frank-Jürgen Methner, Matthias Rädle   +7 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Terahertz and Photoelectron Emission from Nanoporous Gold Films on Semiconductors

Nanomaterials, 2019
Efficient terahertz and photoelectron emission were observed from nano-porous gold (NPG) films deposited on an intrinsic gallium arsenide (GaAs) semiconductor substrate stimulated by femtosecond laser with pulse width of 60 fs.
Junyi Nan, Min Li, Ling Zhang, Shuai Yuan, Boqu He, Heping Zeng   +5 more
doaj   +1 more source

Thermal accommodation in nanoporous silica for vacuum insulation panels

International Journal of Thermofluids, 2020
The thermal accommodation coefficient is a measure for the quality of thermal energy exchange between gas molecules and a solid surface. It is an important parameter to describe heat flow in rarefied gases, for example, in aerospace or vacuum technology.
S. Sonnick, M. Meier, G. Ünsal-Peter, L. Erlbeck, H. Nirschl, M. Rädle   +5 more
doaj   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

FEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito, Haduki Asano, Ayano Ueki, Joe Sakamoto, Yasuhiro Kamei, Hayato Tokumoto, Ayaka Yazawa, Shigeki Kamitani   +7 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Fluorescent and electrochemical paper based dual sensor for simple and low cost detection of human papillomavirus DNA without denaturation

Scientific Reports
Tedious steps for double-stranded DNA (dsDNA) denaturation into single-stranded DNA (ssDNA) are necessary for most DNA samples for probe-based hybridization assays, which complicates the detection process.
Panon Tungkunaruk, Sakda Jampasa, Jutamas Jaewjaroenwattana, Prinjaporn Teengam, Kotchakorn Supabowornsathit, Tirayut Vilaivan, Orawon Chailapakul, Janjira Panchompoo   +7 more
doaj   +1 more source