Results 41 to 50 of about 247,136 (257)

Label-free assessment of complement-dependent cytotoxicity of therapeutic antibodies via a whole-cell MALDI mass spectrometry bioassay

Scientific Reports
Potency assessment of monoclonal antibodies or corresponding biosimilars in cell-based assays is an essential prerequisite in biopharmaceutical research and development.
Stefan Schmidt, Alexander Geisel, Thomas Enzlein, Björn C. Fröhlich, Louise Pritchett, Melanie Verneret, Christian Graf, Carsten Hopf   +7 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

optical spectroscopy

Reviews in Mineralogy and Geochemistry, 2014
Citation: 'optical spectroscopy' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.O04314 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire   +4 more sources

Film Thickness and Glycerol Concentration Mapping of Falling Films Based on Fluorescence and Near-Infrared Technique

Micromachines, 2022
Falling film evaporation processes involve high fluid velocities with continuous variations in local film thickness, fluid composition, and viscosity.
Isabel Medina, Stephan Scholl, Matthias Rädle   +2 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Static characteristics of CMOS digital circuit based on transition metal dichalcogenide transistors

AIP Advances, 2019
Static characteristics of digital combinational logic circuits and Schmitt triggers based on two-dimensional (2D) transition metal dichalcogenides (TMDs) have been systematically explored.
F. F. Mao, Z. Jin, L. Y. Shang, Z. G. Hu, J. H. Chu   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Novel Multimodal Imaging System for High-Resolution and High-Contrast Tissue Segmentation Based on Chemical Properties

Sensors
Accurate and detailed tissue characterization is a central goal in medical diagnostics, often requiring the combination of multiple imaging modalities.
Björn van Marwick, Felix Lauer, Felix Wühler, Miriam Rittel, Carmen Wängler, Björn Wängler, Carsten Hopf, Matthias Rädle   +7 more
doaj   +1 more source

Thermal stability of emission from single InGaAs/GaAs quantum dots at the telecom O-band

Scientific Reports, 2020
Single-photon sources are key building blocks in most of the emerging secure telecommunication and quantum information processing schemes. Semiconductor quantum dots (QD) have been proven to be the most prospective candidates.
Paweł Holewa, Marek Grzegorz Mikulicz, Anna Musiał, Nicole Srocka, David Quandt, André Strittmatter, Sven Rodt, Stephan Reitzenstein, Grzegorz Sęk   +8 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source