Results 71 to 80 of about 383,407 (335)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Switching of three-dimensional optical cages using spatial coherence engineering
APL PhotonicsPrecisely capturing and manipulating microparticles is the key to exploring microscopic mysteries. Optical tweezers play a crucial role in facilitating these tasks. However, existing optical tweezers are limited by their dependence on specific beam modes,
Ying Xu +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Design of Optical Tunnel Switching Networks for Big Data Applications
Applied Sciences, 2020 In this paper, we proposed large-scale optical tunnel switching networks based on the Torus topology network with WSS (Wavelength Selective Switch) for future big data applications.
Yuh-Jiuh Cheng +3 more
doaj +1 more source
An ultra fast optical header replacement technology and its application for broadband optical packet switch. [PDF]
, 2001 An optical header replacement technology based on Self Electro-optic Effect Devices (SEEDs) is presented. By using the measurement result of a 75 μm long SEED device, we simulate an 8 Gbps throughput is achievable.
Beuwer, Wim, Etten, Wim van, Tian, Bo
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Organization of the channel-switching process in parallel computer systems based on a matrix optical switch [PDF]
After a classification and analysis of electronic and optoelectronic switching devices, the design principles and structure of a matrix optical switch is described.
Golomidov, Y. V. +3 more
core +1 more source
Coherent control of light interaction with graphene
, 2014 We report the experimental observation of all-optical modulation of light in a graphene film. The graphene film is scanned across a standing wave formed by two counter-propagating laser beams in a Sagnac interferometer.
Faccio, Daniele +4 more
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source