Results 91 to 100 of about 169,741 (316)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Multiple scattering of light in optical diagnostics of dense sprays and other complex turbid media [PDF]

, 2006
Sprays and other industrially relevant turbid media can be quantitatively and qualitatively characterized using modern optical diagnostics. However, current laser based techniques generate errors in the dense region of sprays due to the multiple ...
Berrocal, Edouard
core  

Optical Communication Performance of Cylindrical Vector Partially Coherent Laguerre–Gaussian Beams in Atmospheric Turbulence

Photonics
The optical communication performance of cylindrical vector partially coherent Laguerre–Gaussian (PCLG) beams in different atmospheric turbulence models are investigated.
Meng Liu, Linxuan Yao, Yaru Gao, Hui Zhang, Yangsheng Yuan, Bohan Guo, Huimin Shi   +6 more
doaj   +1 more source

Spin-Independent Plasmonic Lens

Nanoscale Research Letters, 2019
For the semicircular plasmonic lens, the spiral phase is the origin of the spin-dependent surface plasmon polariton (SPP) focusing. By counterbalancing the spin-dependent spiral phase with another spiral phase or Pancharatnam-Berry phase, we realized the
Guoqun Li, Yuqing Sun, Sen Wang
doaj   +1 more source

Enhanced neuroimaging with a calcium sensor in ex-vivo Drosophila melanogaster brains using closed-loop adaptive optics light-sheet fluorescence microscopy. [PDF]

J Biomed Opt, 2023
Hubert A, Farkouh G, Harms F, Veilly C, Imperato S, Mercier M, Loriette V, Rouyer F, Fragola A.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Full polarization and high coherence control of thermal emissions via saddle-band dispersion engineering

Nature Communications
Photonic engineering in metasurfaces has enabled unprecedented control over thermal emissions in recent years. Here, we present a design strategy that achieves full and simultaneous control over both polarization and coherence of thermal emission across ...
Kaili Sun, Guangdong Wang, Wenyu Li, Yinghan Wang, Yangjian Cai, Lujun Huang, Andrea Alù, Zhanghua Han   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Challenges Faced by Optics

, 2015
Optics is the branch of physics that deals with visible light and other electromagnetic waves. Light has a “dual personality” since it sometimes behaves like a wave and other times like corpuscles or discrete packets of energy called photons.
Beléndez, Augusto, Beléndez Vázquez, Augusto   +1 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source