Results 41 to 50 of about 8,399 (255)
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Iterative thresholding for sparse approximations
, 2008 Sparse signal expansions represent or approximate a signal using a small number of elements from a large collection of elementary waveforms. Finding the optimal sparse expansion is known to be NP hard in general and non-optimal strategies such as ...
Blumensath, T. +3 more
core +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
A rational deferred correction approach to parabolic optimal control problems [PDF]
, 2016 The accurate and efficient solution of time-dependent PDE-constrained optimization problems is a challenging task, in large part due to the very high dimension of the matrix systems that need to be solved.
Stefan Güttel +5 more
core +1 more source
Optimal Derivative-Free Root Finding Methods Based on Inverse Interpolation
Mathematics, 2019 Finding a simple root for a nonlinear equation f ( x ) = 0 , f : I ⊆ R → R has always been of much interest due to its wide applications in many fields of science and engineering.
Moin-ud-Din Junjua +2 more
doaj +1 more source
Iterative optimization calibration method for stereo deflectometry
Optics Express, 2015 An accurate system calibration method is presented in this paper to calibrate stereo deflectometry. A corresponding iterative optimization algorithm is also proposed to improve the system calibration accuracy. This merges CCD parameters and geometrical relation between CCDs and the LCD into one cost function.
Hongyu, Ren, Feng, Gao, Xiangqian, Jiang
openaire +2 more sources
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Mathematics, 2023 A nonlinear equation f(x)=0 is mathematically transformed to a coupled system of quasi-linear equations in the two-dimensional space. Then, a linearized approximation renders a fractional iterative scheme xn+1=xn−f(xn)/[a+bf(xn)], which requires one ...
Chein-Shan Liu +2 more
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source