Results 61 to 70 of about 3,624,996 (285)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Explicit memory schemes for evolutionary algorithms in dynamic environments [PDF]

, 2007
Copyright @ 2007 Springer-VerlagProblem optimization in dynamic environments has atrracted a growing interest from the evolutionary computation community in reccent years due to its importance in real world optimization problems.
Yang, S, Shengxiang Yang, Yang, Shengxiang   +2 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Instance-Optimal Geometric Algorithms [PDF]

Journal of the ACM, 2009
We prove the existence of an algorithm A for computing 2D or 3D convex hulls that is optimal for every point set in the following sense: for every sequence σ of n points and for every algorithm A ′ in a certain class A ...
Peyman Afshani, Jérémy Barbay, Timothy M. Chan   +2 more
openaire   +7 more sources

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

On the use of biased-randomized algorithms for solving non-smooth optimization problems

, 2020
Soft constraints are quite common in real-life applications. For example, in freight transportation, the fleet size can be enlarged by outsourcing part of the distribution service and some deliveries to customers can be postponed as well; in inventory ...
Juan A.A., Corlu C.G., de la Torre R., Tordecilla R.D., Ferrer A.   +4 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Ergonomic Chair Design by Fusing Qualitative and Quantitative Criteria using Interactive Genetic Algorithms [PDF]

, 2008
This paper emphasizes the necessity of formally bringing qualitative and quantitative criteria of ergonomic design together, and provides a novel complementary design framework with this aim. Within this framework, different design criteria are viewed
Ramsden, Jeremy, Tiwari, Ashutosh, Takagi, Hideyuki, タカギ, ヒデユキ, 髙木, 英行, Alexandra Melike Brintrup, Brintrup, Alexandra Melike, Ramsden, Jeremy J.   +7 more
core   +2 more sources

Comparison of Evolutionary Optimization Algorithms for FM-TV Broadcasting Antenna Array Null Filling [PDF]

, 2015
Broadcasting antenna array null filling is a very challenging problem for antenna design optimization. This paper compares five antenna design optimization algorithms (Differential Evolution, Particle Swarm, Taguchi, Invasive Weed, Adaptive Invasive ...
Bruce, Mehrdadi, Aristotelis, Bizopoulos, Emmanouil, Tziris, Zaharias, Zaharis, John, Cosmas, Ian, Glover, Violeta, Holmes, Pavlos, Lazaridis   +7 more
core   +1 more source