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Polydispersity of Serratia marcescens nuclease at optimum pH [PDF]

, 1999
Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the enzyme activity.
Filimonova M., Benedik M., Urazov N., Leshchinskaya I.   +3 more
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The pH Optimum of Human 5-Nucleotidases

Enzymologia biologica et clinica, 2017
The pH optimum of 5-nucleotidase from human thyroid, liver and aorta has been studied. Serum 5NT from patients with hepatobiliary obstruction has also been studied. Methods for the partial purification of thyroid 5NT and for the separation of alkaline phosphatase and 5NT in liver and serum are presented.
P G, Hill, H G, Sammons
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Electrochemical consideration on the optimum pH of bilirubin oxidase

Analytical Biochemistry, 2007
Steady-state current-potential curves were obtained for the direct electron transfer (DET) of bilirubin oxidase (BOD) at a highly oriented pyrolytic graphite electrode, and the theoretical analysis based on nonlinear regression enabled us to determine the formal redox potential (E degrees') of BOD in a wide pH range of 2.0 to 8.5.
Kaori, Otsuka, Takayasu, Sugihara, Yoshio, Tsujino, Toshiyuki, Osakai, Eiichi, Tamiya   +4 more
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Optimum pH and Iodine Absorption of an Enzyme Molecule

Nature, 1952
IN a previous communication it has been shown by Basu and Nandi1 that the extent of inactivation consequent on the unfolding of the protein chain of an enzyme molecule could be detected by iodimetric titration of the SH-groups uncovered by the unfolding of the coiled-up molecules.
S, BASU, S P, NANDI
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Searching of Predictors to Predict pH Optimum of Cellulases

Applied Biochemistry and Biotechnology, 2011
The optimal working conditions for enzymes are very much elegant, and their determination is often through experimental approach, which generally is costly and time-consuming. Therefore, it is important to develop methods to use as simple as possible information to predict the optimal working condition for enzymes.
Shaomin, Yan, Guang, Wu
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pH optimum for the rate of acetylcholine action on neurons

Agents and Actions, 1971
The influence of external pH shifts in the range pH 4.5 to 10 on acetylcholine-induced currents was investigated under voltage clamp conditions in neurons of ‘Helix pomatia’. Observations were limited to neurons which responded to electrophoretic application of acetylcholine with a transient hyperpolarization.
B B, Dunning, X, Machne
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Concerning the pH optimum of peptic hydrolysis

Archives of Biochemistry and Biophysics, 1955
Abstract The significance of hydrogen-ion concentration to peptic hydrolysis of a number of proteins has been studied in order to throw light upon certain aspects of the activity vs. pH curves. Various denatured substrates are readily hydrolyzed within a wide pH interval.
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A new method for the adaptive determination of optimum pH and temperature

Biotechnology and Bioengineering, 1989
AbstractAn adaptive control algorithm for the on‐line determination of optimal temperature or pH for biomass production in a continuous fermentor is presented. The algorithm requires no prior information and uses a dynamic Hammerstein model to identify parameters and to estimate an optimal steady‐state control value.
J L, Harmon, G, Lyberatos, S A, Svoronos
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Enzymes in preparative organic synthesis: Coincidence of the pH optimum for catalyst effectiveness with the pH optimum for the catalyzed reaction equilibrium

Biotechnology and Bioengineering, 1981
AbstractThe study concerned the pH profile of the apparent equilibrium constant for synthesis of N‐benzoyl‐L‐phenylalanine ethyl ester from the respective acid and ethanol in the biphasic system chloroform + 5% (v/v) water. The substitution of water (as a reaction medium) for the biphasic aqueous–organic system shifted the pH profile toward neutral pH ...
Karel Martinek, Anatole N. Semenov, Ilya V. Berezin   +2 more
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Optimum pH for nuclear sex identification using quinacrine

Clinical Genetics, 1975
Preparations of quinacrine stained interphase nuclei from buccal smears and hair root sheaths were mounted in Macllvaine's buffer at various pH's in an attempt to obtain optimum differentiation of X‐ and Y‐chromatin. Relatively high pH (5–8) was associated with intense nuclear fluorescence. Background nuclear fluorescence decreased with lower pH's (2–4)
B R, Korf, B E, Schuh, M J, Salwen
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