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Searching of Predictors to Predict pH Optimum of Cellulases
Applied Biochemistry and Biotechnology, 2011The optimal working conditions for enzymes are very much elegant, and their determination is often through experimental approach, which generally is costly and time-consuming. Therefore, it is important to develop methods to use as simple as possible information to predict the optimal working condition for enzymes.
Shaomin, Yan, Guang, Wu
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Optimum pH and Iodine Absorption of an Enzyme Molecule
Nature, 1952IN a previous communication it has been shown by Basu and Nandi1 that the extent of inactivation consequent on the unfolding of the protein chain of an enzyme molecule could be detected by iodimetric titration of the SH-groups uncovered by the unfolding of the coiled-up molecules.
S, BASU, S P, NANDI
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Optimum composition of neutral carrier based pH electrodes
Analytica Chimica Acta, 1994Abstract A simple formalism for the quantitative description of the upper and lower detection limit of pH selective solvent polymeric membrane electrodes containing a neutral carrier and a lipophilic anionic additive is presented, which is based on the consideration of phase transfer equilibria at the sample/membrane interface.
Eric Bakker, Aiping Xu, Ernö Pretsch
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pH optimum for the rate of acetylcholine action on neurons
Agents and Actions, 1971The influence of external pH shifts in the range pH 4.5 to 10 on acetylcholine-induced currents was investigated under voltage clamp conditions in neurons of ‘Helix pomatia’. Observations were limited to neurons which responded to electrophoretic application of acetylcholine with a transient hyperpolarization.
B B, Dunning, X, Machne
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Upward shift of the pH optimum of Acremonium ascorbate oxidase
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2002A gene encoding a thermostable Acremonium ascorbate oxidase (ASOM) was randomly mutated to generate mutant enzymes with altered pH optima. One of the mutants, which exhibited a significantly higher activity in the pH range 4.5-7 compared to ASOM, had a Gln183Arg substitution in the region corresponding to SBR1, one of the substrate binding regions of ...
Masayasu, Sugino +7 more
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Nitrate reductase from Penicilliumchrysogenum: Kinetic mechanism at sub-optimum pH
Biochemical and Biophysical Research Communications, 1982Abstract The steady-state kinetics of the NADPH + FAD-dependent reduction of nitrate by nitrate reductase from Penicillium chrysogenum was studied at pH 6.18. At this sub-optimum pH, Vmax was about 83 units × mg protein−1 compared with 225 units × mg protein−1 at pH 7.20.
F, Renosto, N D, Schmidt, I H, Segel
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Journal of Physical Chemistry C, 2019
The wet chemical processes of LiFePO4, hydrothermal synthesis and hydrometallurgical recovery, are of great importance during the life cycle of LiFePO4. To analyze these two processes, E-pH diagrams for the Li-Fe-P-H2O system are plotted from 298 to 473 ...
Qi Jing +6 more
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The wet chemical processes of LiFePO4, hydrothermal synthesis and hydrometallurgical recovery, are of great importance during the life cycle of LiFePO4. To analyze these two processes, E-pH diagrams for the Li-Fe-P-H2O system are plotted from 298 to 473 ...
Qi Jing +6 more
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Polydispersity of Serratia marcescens nuclease at optimum pH
1999Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the enzyme activity.
Filimonova M. +3 more
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pH optimum of the reduction of dehydroascorbic acid by dithioerytritol
Scandinavian Journal of Clinical and Laboratory Investigation, 1993Ascorbic acid is a water soluble antioxidant, that is itself oxidized to dehydroascorbic acid. In order to detect dehydroascorbic acid by amperometri it has to be reduced to ascorbic acid beforehand. This reduction was performed with dithioerytritol at pH = 6.0 for 10 min, which is a choice between optimal reaction rate and stability of ascorbic acid ...
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Shifting pH optimum of Bacillus circulans xylanase based on molecular modeling
Journal of Biotechnology, 2008Although hydrolases are used in several industrial processes, its industrial applications have some limitations in specific cases since some industrial processes are carried out at pH value which is different from optimum pH of the enzyme. Alkaline side optimum pH of hydrolases is always desirable, and it is proved difficult to achieve that by mutation
Ji Hye, Yang +3 more
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