Results 141 to 150 of about 300,025 (308)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

open access: yesFEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão   +3 more
wiley   +1 more source

Studies on the Optimum Temperature (Rep. 1.) : On the Optimum Temperature of Air-Conditioning in Summer

open access: yesSangyo Igaku, 1960
T. MIURA   +5 more
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Studi Numerik Karakteristik Perpindahan Panas pada Membrane Wall Tube Boiler Dengan Variasi Jenis Material dan Ketebalan Insulasi di PLTU Unit 4 PT.PJB UP Gresik

open access: yesJurnal Teknik ITS, 2016
Abstrak-Salah satu komponen terpenting dalam siklus pembangkit listrik bertenaga uap adalah boiler, dimana fungsi utama dari komponen ini adalah penghasil steam (uap) yang akan menggerakan turbin guna menghasilkan tenaga listrik.
I Nyoman Ari Susastrawan D, Prabowo - -
doaj  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

Uji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 Isolat Asal Limbah Cairan Rumen Sapi Peranakan Ongole (GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAE SAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CRO

open access: yesJurnal Veteriner, 2016
This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask ...
Tri Nurhajati   +2 more
doaj  

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

open access: yesFEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo   +6 more
wiley   +1 more source

Map 2 - Holocene Climatic Optimum (8 ± 1 ka cal BP) average surface air temperature of about 2.5 °C higher than today.

open access: yes, 2004
Thematic map of Holocene climatic Optimum, with informations on glaciers, vertebrates, serpulids, lakes, vegetation, alluvial plains, Apennine foothills, marine bathymetry and bottom morphology, shore lines, sea surface temperature, archaeology, active ...
GIARDINA F. ACTIVE FAULTS   +31 more
core  

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