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A simple and reliable method for determination of optimum pH in coupled enzyme assays [PDF]

open access: yesBioTechniques, 2020
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.
Lee Bowman   +7 more
doaj   +4 more sources

Soil pH Range for Optimum Commercial Vegetable Production

open access: yesEDIS, 2012
Soil pH affects nutrient bioavailability by controlling the chemical forms of nutrients. This 11-page fact sheet provides information about soil pH basics to commercial growers, county Extension agents, and college students specializing in vegetable ...
Guodong Liu, Edward Hanlon
doaj   +7 more sources

On the pH‐optimum of activity and stability of proteins [PDF]

open access: yesProteins: Structure, Function, and Bioinformatics, 2010
AbstractBiological macromolecules evolved to perform their function in specific cellular environment (subcellular compartments or tissues); therefore, they should be adapted to the biophysical characteristics of the corresponding environment, one of them being the characteristic pH.
Kemper, Talley, Emil, Alexov
openaire   +3 more sources

Obtaining L - Glutamate Oxidase Enzyme from Streptomyces and Estimation Its Toxicity on Blood Components [PDF]

open access: yesJournal of Applied Sciences and Nanotechnology, 2021
Streptomyces was isolated from the soil and then tested to diagnose morphologically and microscopically. Also, cultivated in the production medium of the L-glutamate oxidase enzyme, for which the wheat bran culture media was used, and then the ...
Dina Abdul Hameed, Entesar Ali
doaj   +1 more source

Investigation of Nitrate Removal from Aqueous Solutions by Egg Shell Nanostructure Adsorbent [PDF]

open access: yesمحیط زیست و مهندسی آب, 2019
Pollution of groundwater and surface water into nitrate in many parts of the world is a serious problem. Nitrate can cause Eutrophication water sources and related problems.
Ali Bafkar, Neda Babeli
doaj   +1 more source

Engineering the optimum pH of β-galactosidase from Aspergillus oryzae for efficient hydrolysis of lactose

open access: yesJournal of Dairy Science, 2022
: β-Galactosidase (lacA) from Aspergillus oryzae is widely used in the dairy industry. Its acidic pH optimum and severe product inhibition limit its application for lactose hydrolysis in milk.
Xin Shi, Dan Wu, Yan Xu, Xiaowei Yu
doaj   +1 more source

MCIC: Automated Identification of Cellulases From Metagenomic Data and Characterization Based on Temperature and pH Dependence

open access: yesFrontiers in Microbiology, 2020
As the availability of high-throughput metagenomic data is increasing, agile and accurate tools are required to analyze and exploit this valuable and plentiful resource.
Mehdi Foroozandeh Shahraki   +7 more
doaj   +1 more source

Potensi Cangkang Telur Ayam dan Cangkang Telur Bebek sebagai Bioadsorben Logam Pb dari Limbah Cair Industri Farmasi

open access: yesKovalen: Jurnal Riset Kimia, 2022
The pharmaceutical industry liquid waste is one of the wastes that have the potential to produce waste containing heavy metals. One of the highest metal contaminants originating from the pharmaceutical industry is lead (Pb).
Lisna Dewi   +2 more
doaj   +1 more source

Karakterisasi ekstrak kasar fitase termofilik dari bakteri kawah Ijen Banyuwangi, isolat AP-17

open access: yesJournal of Biological Researches, 2010
Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17.
Aline Puspita Kusumadjaja   +3 more
doaj   +1 more source

The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum [PDF]

open access: yesJournal of the Korean Chemical Society, 2002
본 논문에서는 산화 환원 효소의 최적 pH를 예측해 보았다. Boltzman 분배를 이용하여 어떤 아미노산의 side chain이 물에서 발견될수 있는 상대적 확률이나 물에 대한 상대적 친화력을 구하였으며 p $K_R$ 을 이용해 protonated 아미노산의 양과 deprotonated 아미소산의 양을 계산하였다. 효소의 최적 pH는 아미노산의 side chain이 물에서 발견될수 있는 상대적 확률과 protonated 또는 deprotonated된 아미노산 양의 곱인 유효 protonated된 양과 유효 depotonated된 양이 같아지는 pH로 예측하였다. 문헌 값과 비교해 보았을 때 예측치는 상당히 일치하는 경향을
openaire   +1 more source

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