Results 21 to 30 of about 208,937 (301)
On the Optimum pH for Mold-Lecithase
NIPPON SUISAN GAKKAISHI, 1932 AKAMATSU found by the estimation of the increased acidity and of the inorganic phosphoric acid that Taka-phosphatase (which is contained in Taka-diastase from Aspergillus oryzae) decomposes not only the synthetical glycerin-phosphoric acid, but also the glycerin-phosphoric acid which is a decomposed product from lecithin.
OYA, Takeo, KIMATA, Masao
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Predicting pH Optimum for Activity of Beta-Glucosidases [PDF]
Journal of Biomedical Science and Engineering, 2019 The working conditions for enzymatic reaction are elegant, but not many optimal conditions are documented in literatures. For newly mutated and newly found enzymes, the optimal working conditions can only be extrapolated from our previous experience. Therefore a question raised here is whether we can use the knowledge on enzyme structure to predict the
Shaomin Yan, Guang Wu
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Acta Scientiarum: Technology, 2013 The enzyme cyclomaltodextrin-glucanotransferase (CGTase) is a transglicosidase able to convert corn starch into cyclodextrin (CD). CDs are widely applied in industry given the ability to form inclusion complexes with a great variety of organic molecules.
Jéssica Bravin Carmello +2 more
doaj +1 more source
Machine learning prediction of enzyme optimum pH
Nature Machine Intelligence, 2023 Abstract The relationship between pH and enzyme catalytic activity, especially the optimal pH (pHopt) at which enzymes function, is critical for biotechnological applications. Hence, computational methods to predict pHopt will enhance enzyme discovery and design by facilitating accurate identification of enzymes that function optimally ...
Japheth E. Gado +6 more
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Metal dependence and optimum pH.
, 2013 Phosphatase activity was measured using p-NPP. A) Magnesium dose-dependent increases of activities. B) Optimum pH determinations. Three different buffers were used: Sodium acetate at pH 4.5 to 5.5, MES at pH 5.5 to 7 and Tris at pH 7 to 9.
Jaime G. Mayoral (441569) +5 more
core +1 more source
Characterization of phospholipase B of Culex pipiens fatigans
Journal of Lipid Research, 1969 Phospholipase B has been found in the mosquito Culex pipiens fatigans, and some of its properties have been studied. The enzyme had a high optimum temperature (45°C) and broad alkaline pH optimum (8-9).
R. Hanumantha Rao, D. Subrahmanyam
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Therapeutic Apheresis and Dialysis, EarlyView.ABSTRACT Background Neuromyelitis optica spectrum disorder (NMOSD) is a relapsing autoimmune disease of the central nervous system. High‐dose intravenous methylprednisolone (IVMP) is the standard first‐line therapy for acute attacks, although some patients remain refractory.
Wataru Horiguchi +5 more
wiley +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
The pH optimum and pH stability of rOs1BGlu4 hydrolysis activity.
, 2014 A. pH optimum determination: rOs1BGlu4 (0.25 µg) was assayed with 1 mM pNPGlc in different 50 mM pH buffers (formate, pH 4.0; sodium acetate, pH 4.5–5.5; sodium phosphate, pH 6.0–7.5; Tris, pH 8.0–9.5; CAPS, pH 10.0–11.0) at 30°C for 10 min. B.
Supaporn Baiya (560011) +6 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source