Results 121 to 130 of about 4,446,877 (297)
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Optimum Currency Areas theory and the Slovak suitability for the euro adoption [PDF]
This paper tests the present readiness of Slovakia to join the European Monetary Union (EMU) based on the classical Optimum Currency Area criteria, the degree of trade intensity and business cycle synchronization.
DomeNico Raguseo, Jan Sebo
core
The optimum temperature for the heat therapy for meibomian gland dysfunction. [PDF]
Ocul Surf, 2019 Borchman D.
europepmc +1 more source
Nigerian Journal of Technological Development, 2020 Pinch technology is one of the most powerful methodologies of process integration that allows industries to increase their profitability through reductions in energy, water and raw materials consumption. In this study, reduction in the total annual cost
Abdulkareem Abubakar
doaj
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
A Theory of Optimum Tariff Under Revenue Constraint. [PDF]
This paper analyzes the revenue-constrained optimum tariff problem. When a fixed level of tax revenue has to be collected only from tariffs, an efficient resource allocation can not be achieved by any tariff structure.
Ogawa, Y., hatta, T.
core
Effect of assay duration on the optimum enzyme temperature.
, 2019 A) Relative activity of Sfβgly during the assays at different temperatures. (purple cross) 29°C; (blue circle) 33°C; (blue diamond) 37°C; (red square) 42°C; (green triangle) 46°C.
Sandro R. Marana (560928) +1 more
core +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source