Results 111 to 120 of about 4,446,877 (297)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

open access: yesFEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Thermal Properties of Two Materials Commonly Usedin Low Temperature Laboratories [PDF]

open access: yes, 2013
We carried out measurements of thermal conductance and thermal contact resistance of two materials commonly used in low temperature laboratories such as an Electro-Magnetic Interference (EMI) Filter and Stycast 2850 FT epoxy.
ANGEL ENRIQUE SANCHEZ COLIN
core  

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Optimum Choice for Reference Temperature in Evaluation of Curing Cycles

open access: yes, 1979
An exact expression for the optimum reference temperature to be used in evaluating curing cycles is developed. This reference temperature minimizes the dependence of the equivalent cure time on the activation energy.
E. J. Galli, A. L. Chehebar
core   +1 more source

Characterization of Crude Extract of Mucor pusillus Rennin

open access: yesJurnal Ilmu dan Teknologi Hasil Ternak, 2012
The research preparation involved production of rennin, determination of optimum pH of crude extract of rennin, determination of optimum temperature of crude extract of rennin, and determination of enzyme kinetics.
Khothibul Umam Al Awwaly   +2 more
doaj  

Comparative analysis of drying methods and optimization of Teff Injera drying parameters using RSM and SLP approach

open access: yesScientific Reports
The drying process of Teff Injera, a staple and highly favored traditional Ethiopian food made from Teff, was investigated to optimize moisture removal and determine the ideal drying time under controlled conditions.
Dawit Andualem Asrate   +2 more
doaj   +1 more source

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