Combined Transcriptomic and Metabolomic Analysis Reveals an Ethylene‐Activated Regulatory Model on Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus [PDF]
Plant Biotechnology Journal, Volume 24, Issue 2, Page 363-380, February 2026.ABSTRACT Catharanthus roseus contains nearly 200 bioactive monoterpenoid indole alkaloids (MIAs) that are effective in treating cancer and other diseases. Ethylene plays a significant role in enhancing MIA biosynthesis, and we have found that it greatly induces the accumulation of anhydrovinblastine.
Bofu Deng +9 more
wiley +2 more sources
Cell‐type‐aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus [PDF]
New Phytologist, Volume 245, Issue 1, Page 347-362, January 2025.Summary In plants, the biosynthetic pathways of some specialized metabolites are partitioned into specialized or rare cell types, as exemplified by the monoterpenoid indole alkaloid (MIA) pathway of Catharanthus roseus (Madagascar Periwinkle), the source of the anticancer compounds vinblastine and vincristine. In the leaf, the C.
Chenxin Li +8 more
wiley +2 more sources
Explant origin and growth regulators as determinants of callus performance and terpenoid indole alkaloid accumulation in Catharanthus roseus [PDF]
BMC Plant BiologyCatharanthus roseus (L.) G. Don (Apocynaceae) is a highly important medicinal plant and a valuable source of diverse pharmacologically active compounds particularly anticancer terpenoid indole alkaloids (TIAs), such as catharanthine (CAT), vinblastine ...
Abeer F. Desouky +5 more
doaj +2 more sources
A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants [PDF]
Plant MethodsBackground Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies across plant species.
Yongliang Liu +5 more
doaj +2 more sources
Metabolomics analysis reveals both plant variety and choice of hormone treatment modulate vinca alkaloid production in Catharanthus roseus [PDF]
Plant Direct, Volume 4, Issue 9, September 2020., 2020 Abstract The medicinal plant Catharanthus roseus produces numerous secondary metabolites of interest for the treatment of many diseases – most notably for the terpene indole alkaloid (TIA) vinblastine, which is used in the treatment of leukemia and Hodgkin's lymphoma.
Valerie N. Fraser +2 more
wiley +2 more sources
EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings [PDF]
Frontiers in Plant Science, 2019 The Catharanthus roseus plant is the exclusive source of the valuable anticancer terpenoid indole alkaloids, vinblastine (VB) and vincristine (VC). The recent availability of transcriptome and genome resources for C.
Samuel Mortensen +8 more
doaj +2 more sources
Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism [PDF]
New Phytologist, Volume 247, Issue 2, Page 774-790, July 2025.Summary Acylsugars, specialized metabolites produced by trichomes of many solanaceous species, provide protection against biotic and abiotic stresses. Many acylsugar metabolic enzymes have been identified; however, regulatory factors remain unknown.
Sabyasachi Mandal +2 more
wiley +2 more sources
Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings [PDF]
Plant Direct, Volume 8, Issue 6, June 2024.Abstract Agrobacterium‐mediated transient expression methods are widely used to study gene function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified the effect of Agrobacterium‐infiltration parameters on the transient transformation efficiency of Catharanthus roseus seedlings.
Lauren F. Cole‐Osborn +2 more
wiley +2 more sources
Ecology and Evolution, Volume 12, Issue 5, May 2022., 2022 We investigated the genetic basis for the production of triterpenoid saponins in the medicinal plant, Ardisia kteniophylla, using transcriptome sequences and qRT‐PCR. We found that genes involved in late stages of triterpenoid saponin biosynthesis are upregulated in roots, which are traditionally regarded as the medicinal organ of the plant, compared ...
Yuyang Lei +6 more
wiley +1 more source
Biosynthesis of Secondary Metabolites Based on the Regulation of MicroRNAs
BioMed Research International, Volume 2022, Issue 1, 2022., 2022 MicroRNA (miRNA), a noncoding ribonucleic acid, is considered to be important for the progression of gene expression in plants and animals by rupture or translational repression of targeted mRNAs. Many types of miRNA regulate plant metabolism, growth, and response to biotic and abiotic factors.
Rajib Hossain +15 more
wiley +1 more source