Results 41 to 50 of about 665,321 (256)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Effectively Lossless Subspace Appearance Model Compression Using Prior Information

open access: yesTsinghua Science and Technology, 2015
Subspace appearance models are widely used in computer vision and image processing tasks to compactly represent the appearance variations of target objects.
Yang Li, Xiaoming Tao, Jianhua Lu
doaj   +1 more source

Store image through the eyes of the retail manager [PDF]

open access: yesThe Retail and Marketing Review, 2022
The purpose of the research is to identify Retail Managers’ perception regarding store image dimensions including store appearance, frontline staff, merchandise display and in-store experience of customer service by following a secret shopping ...
Prof Johannes A Wiid
doaj  

Design and analysis strategies for robust microbiome ageing research

open access: yesFEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik   +5 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Risk of damage and desiccation cracking of construction materials based on raw earth [PDF]

open access: yesMATEC Web of Conferences, 2019
Nowadays, structures are mainly constructed using natural aggregates as sand and gravels. In the future, we would increasingly have to consider replacing them by more abundant and ecological natural materials such as raw earth.
El Hajjar Ahmad   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

Study on Longjing Tea Grade Identification and Appearance Evaluation Method Based on Image Features and Intelligent Algorithm [PDF]

open access: yesChaye kexue
Longjing tea appearance features are crucial for assessing tea quality. In order to intelligently evaluate the appearance characteristics of Longjing tea and quickly recognize the grades, a method combining image features and intelligent algorithms for ...
HUANG Danyi, ZHAO Zhumeng, FAN Dongmei, LIN Jie, LU Yating, WANG Xiaochang, LI Lamei
doaj   +1 more source

Accurate Recognition of Motion Patterns Based on Artificial Visual Neural Network

open access: yesAdvances in Multimedia, 2022
In order to improve the accuracy of motion pattern recognition, this paper combines the artificial visual neural network to construct a motion pattern recognition system.
Meiqi Li
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

open access: yesFEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang   +4 more
wiley   +1 more source

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