Results 181 to 190 of about 3,808,046 (284)

FECH, a novel metabolic target influencing CAR T-cell phenotype and function. [PDF]

Biomark Res
Pellegrino M, Vespa S, Salvatori I, Mac GD, Valle C, Secli V, Cairoli S, Goffredo BM, Caforio M, Folgiero V, Sanvi LW, Vulpis E, Ciampini E, Scarsella M, Giorda E, Chiaradonna F, Giansanti M, Nazio F, Ferri A, Cecconi F, Campello S, Velardi E, Caruana I, de Billy E.   +23 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A gut commensal <i>Serratia marcescens</i> inhibits dengue virus infection via prodigiosin-induced autophagy in <i>Aedes albopictus</i>. [PDF]

Virulence
Huang Y, Li H, Ding J, Zhu Y, Wang N, Zhang J, Wang H, Feng X, Xu H, Liu W, Liang S.   +10 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Long-term immunity and protection against SARS-CoV-2 XBB.1.5 following homologous and heterologous mRNA and MVA vaccination. [PDF]

Front Immunol
Pérez P, Esteso G, García-García I, Flores S, Sánchez-Corzo C, Noriega MA, Casasnovas JM, Esteban M, García-Arriaza J.   +8 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Anti-inflammatory CAR-microglia targeting Aβ for Alzheimer's disease therapy. [PDF]

Front Immunol
Ding X, Hu X, Xue W, An Y, Cheng W, Zhang S, Lei A, Zhang J.   +7 more
europepmc   +1 more source

Freedom of expression, the double-edged sword

openThis thesis explores how freedom of expression, traditionally a cornerstone of Western democracies, is increasingly instrumentalized by politicians and corporations for their own interests.
LOZANO ARIZA, DANIEL
core  

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Sedanolide alleviates LPS‑induced depressive‑like behaviors by modulating the C3a/C3aR signaling axis and microglial glycolysis. [PDF]

Mol Med Rep
Lu S, Luo F, Chen N, Shi G, Chen Y.
europepmc   +1 more source