Culture of fetal mouse liver in plastic chambers: A new technique for organ culture
A new technique for organ culture which uses plastic culture chambers and the advantages of the cellophane-sheet technique is described with the results of a study of cultivations of fetal mouse liver. Two chambers, each containing cells, were placed in gas permeable roller tubes and rotated at 0.1 rpm in a CO2-air gassed incubator.
Masayoshi Kumegawa+2 more
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Significance of organ culture techniques for evaluation of prenatal toxicity
A discussion of the applicability of in vitro techniques now available for research in prenatal toxicology is presented. Advantages and disadvantages of the various in vitro methods (such as cultivation of preimplantation embryos, whole embryo culture, and organ culture) as applied to various problems of experimental research are described.
Hans-Jürgen Barrach, Diether Neubert
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A TECHNIQUE OF ORGAN CULTURE FOR PROTRACTED METABOLISM STUDIES
The development of a perfusion apparatus capable of sustaining life and growth in an excised organ has occasioned much study. Carrel and Lindbergh (1938) presented the technique as developed by them and their associates at that time. Most recently Long (1946) and Anderson and Long (1947) and Hetcher (1948) have shown the value of an in vitro study of ...
Nicholas T. Werthessen
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Aspects of morphogenesis and cytodifferentiation of endoderm offered by organ culture techniques.
In this article, I will summarize the results obtained in my laboratory from the study of organogenesis since 1970. The results have shown region-specific directive influence of mesenchymes and reactivity of endoderms including those of the allantois, yolk sac, digestive tract and urogenital sinus.
Takuya Mizuno
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Development and evaluation of a porcine in vitro colon organ culture technique
The intestinal mucosa comprises a complex assemblage of specialized tissues that interact in numerous ways. In vitro cell culture models are generally focused on recreating a specific characteristic of this organ and do not account for the many interactions between the different tissues.
Matheus O. Costa+2 more
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Genetic Resistance of Chickens to Aflatoxin Assessed with Organ-Culture Techniques
A chick tracheal-organ-culture model system was used to evaluate the sensitivity of various strains of chickens to aflatoxin B1. Tissues from different genetic stocks showed highly significant differences in in vitro sensitivity to aflatoxin. Tracheal tissues from brown-egg layers were most sensitive, meat-type birds intermediate, and white-egg layers ...
C. M. Williams+2 more
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Development of an Organ Culture Technique Capable of Monitoring Most Pineal Gland Indole Metabolites
An intact pineal gland organ culture technique was developed which utilised radiolabelled tryptophan as the indolic precursor and two‐dimensional thin‐layer chromatography to separate the various indole metabolites produced. The method was capable of reproducibly separating and quantitating all tryptophan metabolites except 5‐methoxytryptophan which co‐
Dougal J. Morton
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A modified technique for organ culture in vitro
O. A. Trowell
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Propagation of Sandalwood (Santalum Album Linn) using Tissue and Organ Culture Technique
A majority of the woody plants are difficult to propagate by vegetative means. Traditional methods such as rooting of the excised branches, and grafting, although successful in some instances, still has limitations and cannot be applied for all commercially valuable species.
P. S. Rao, N. V. Raghava Ram
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Submerged organ culture technique
Theodore Fainstat
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