Techniques for tissue culture have seen significant advances during the last decades and novel 3D cell culture systems have become available. To control their high complexity, experimental techniques and their Digital Twins (modelling and computational ...
Ehsan Fattahi+4 more
doaj +1 more source
3D Cell Cultures: Evolution of an Ancient Tool for New Applications
Recently, research is undergoing a drastic change in the application of the animal model as a unique investigation strategy, considering an alternative approach for the development of science for the future.
Andrea Cacciamali+2 more
doaj +1 more source
Shoot organogenesis in leaf explants of Hydrangea macrophylla ‘Hyd1’ and assessing genetic stability of regenerants using ISSR markers [PDF]
For the first time, an in vitro regeneration protocol of Hydrangea macrophylla 'Hyd1' was developed. Effects of different plant growth regulators (PGRs) on shoot regeneration were investigated jointly with selecting optimal basal media and cefotaxime ...
Huang, L.L.+4 more
core +2 more sources
In vitro propagation and homing of liver-derived dendritic cell progenitors to lymphoid tissues of allogeneic recipients: Implications for the establishment and maintenance of donor cell chimerism following liver transplantation [PDF]
Dendritic cell (DC) progenitors were propagated in liquid culture from nonparenchymal cells resident in normal mouse (B10.BR; H-2k, I-E+) liver in response to granulocyte-macrophage colony stimulating factor (GM-CSF).
Fung, JJ+7 more
core +1 more source
High-throughput design of cultured tissue moulds using a biophysical model [PDF]
The technique presented here identifies tethered mould designs, optimised for growing cultured tissue with very highly-aligned cells. It is based on a microscopic biophysical model for polarised cellular hydrogels. There is an unmet need for tools to assist mould and scaffold designs for the growth of cultured tissues with bespoke cell organisations ...
arxiv
In vitro spermatogenesis: A century‐long research journey, still half way around
Background Spermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro.
Mitsuru Komeya+2 more
doaj +1 more source
In vitro strategies for the enhancement of secondary metabolite production in plants: a review
Background Plants are the prime source of vital secondary metabolites (SMs) which are medicinally important for drug development, and these secondary metabolites are often used by plants in the various important tasks like defense against herbivory ...
Mohammad Afaan Fazili+4 more
doaj +1 more source
Human natural killer cell committed thymocytes and their relation to the T cell lineage. [PDF]
Recent studies have demonstrated that mature natural killer (NK) cells can be grown from human triple negative (TN; CD3-, CD4-, CD8-) thymocytes, suggesting that a common NK/T cell precursor exists within the thymus that can give rise to both NK cells ...
Lanier, LL+3 more
core +1 more source
Corporate Culture and Organizational Fragility [PDF]
Complex organizations accomplish tasks through many steps of collaboration among workers. Corporate culture supports collaborations by establishing norms and reducing misunderstandings. Because a strong corporate culture relies on costly, voluntary investments by many workers, we model it as an organizational public good, subject to standard free ...
arxiv
Hematopoietic progenitor cell content of vertebral body marrow used for combined solid organ and bone marrow transplantation [PDF]
While cadaveric vertebral bodies (VB) have long been proposed as a suitable source of bone marrow (BM) for transplantation (BMT), they have rarely been used for this purpose. We have infused VB BM immediately following whole organ (WO) transplantation to
Ball, ED+6 more
core +1 more source