Results 11 to 20 of about 1,806 (131)

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Microbial Biotechnology, 2020
Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species.
Ke Liu, Bin Sun, Hao You, Jun‐Liang Tu, Xuya Yu, Peng Zhao, Jun‐Wei Xu   +6 more
doaj   +1 more source

Activity‐Based Protein Profiling in Methicillin‐Resistant Staphylococcus aureus Reveals the Broad Reactivity of a Carmofur‐Derived Probe

ChemBioChem, Volume 24, Issue 21, November 2, 2023., 2023
Enzyme targets of activity‐based probes built on the anti‐neoplastic drug and antimicrobial agent carmofur in methicillin‐resistant Staphylococcus aureus (MRSA) have been identified. Chemoproteomic profiling revealed a broad reactivity of the probe against both cysteine and serine hydrolases, and identified uncharacterized bacterial enzymes; this has ...
Md Jalal Uddin, Hermen S. Overkleeft, Christian S. Lentz   +2 more
wiley   +1 more source

Cloning and Overexpressing Membrane Proteins Using Pichia pastoris (Komagataella phaffii)

Current Protocols, Volume 3, Issue 11, November 2023., 2023
Abstract Understanding the structure and function of key proteins located within biological membranes is essential for fundamental knowledge and therapeutic applications. Robust cell systems allowing their actual overexpression are required, among which stands the methylotrophic yeast Pichia pastoris.
Magali Schwob, Valérie Kugler, Renaud Wagner   +2 more
wiley   +1 more source

Integrated genomics and phenotype microarray analysis of Saccharomyces cerevisiae industrial strains for rice wine fermentation and recombinant protein production

Microbial Biotechnology, Volume 16, Issue 11, Page 2161-2180, November 2023., 2023
The integrated genomics and phenotype microarray analysis of industrial S. cerevisiae strains elucidated their evolutionary history and genomic elements responsible for metabolic and physiological characteristics associated with biotechnical applications.
Ye Ji Son, Min‐Seung Jeon, Hye Yun Moon, Jiwon Kang, Da Min Jeong, Dong Wook Lee, Jae Ho Kim, Jae Yun Lim, Jeong‐Ah Seo, Jae‐Hyung Jin, Yong‐Sun Bahn, Seong‐il Eyun, Hyun Ah Kang   +12 more
wiley   +1 more source

Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recycling

Molecular Plant Pathology, Volume 24, Issue 11, Page 1451-1464, November 2023., 2023
We established an efficient multiple gene disruption method in Colletotrichum higginsianum utilizing CRISPR/Cas9 and a recyclable marker. Abstract Colletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana.
Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean‐Félix Dallery, Richard O'Connell, Ken Shirasu   +6 more
wiley   +1 more source

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

BMC Microbiology, 2020
Background ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure.
Jinxin Zheng, Yang Wu, Zhiwei Lin, Guangfu Wang, Sibo Jiang, Xiang Sun, Haopeng Tu, Zhijian Yu, Di Qu   +8 more
doaj   +1 more source

Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology

Applied Research, Volume 2, Issue 5, October 2023., 2023
We present a genome engineering approach of Aspergillus oryzae NSAR1 using in vitro assembled Cas9:gRNA ribonucleoproteins. This method allowed for genome editing in three distinct ways. First, insertion or deletion of bases (INDELs) were generated by using the ribonucleoproteins without repair template. This was used for disruption of the pyrG gene to
Gerrit Haeger, Alina Grankin, Michaela Wagner   +2 more
wiley   +1 more source

Role of 6‐phosphogluconate dehydrogenase enzyme 1 in growth and virulence of Toxoplasma gondii and development of attenuated live vaccine

Microbial Biotechnology, Volume 16, Issue 10, Page 1957-1970, October 2023., 2023
Tg6PGDH1 in the oxidative pentose phosphate pathway is a cytoplasmic protein that is dispensable for tachyzoite growth of Toxoplasma gondii in vitro but critical for virulence and cyst formation in vivo. ME49Δ6pgdh1 immunization stimulated strong protective immunity against lethal challenges and blocked cyst formation caused by reinfection.
Qinghong Guo, Xuefang Guo, Nuo Ji, Bang Shen, Xinhua Zhong, Lihua Xiao, Yaoyu Feng, Ningbo Xia   +7 more
wiley   +1 more source

Inhibitors of Aspartate Transcarbamoylase Inhibit Mycobacterium tuberculosis Growth

ChemMedChem, Volume 18, Issue 17, September 1, 2023., 2023
Allosteric inhibitors designed to bind to the malarial aspartate transcarbamoylase (ATCase) and inhibit pyrimidine biosynthesis are shown to also inhibit the ATCase of M. tuberculosis. The most potent of this series also inhibit proliferation of M. tuberculosis in cell culture.
Xiaochen Du, Vidhisha Sonawane, Bidong Zhang, Chao Wang, Bram de Ruijter, Alexander S. S. Dömling, Norbert Reiling, Matthew R. Groves   +7 more
wiley   +1 more source

Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic Mucorales Mucor circinelloides

G3: Genes, Genomes, Genetics, 2017
Mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited.
Alexis Garcia, Gloria Adedoyin, Joseph Heitman, Soo Chan Lee   +3 more
doaj   +1 more source