Results 11 to 20 of about 4,091 (157)

Exploring the Causal Relationship Between Blood Metabolites and Chronic Periodontitis: Insights From Genetic Causal Analysis. [PDF]

J Cell Mol Med
ABSTRACT To investigate the potential bidirectional causal relationship between specific blood metabolites and chronic periodontitis using Mendelian randomisation (MR). A two‐sample bidirectional MR analysis was conducted. Data on 1400 blood metabolites were obtained from a genome‐wide association study involving 8299 participants.
Cai W, Zhao H, Wang P, Chen X, Yang Y, Wu H, Chen Z, Fang F, Qiu W.   +8 more
europepmc   +2 more sources

In Silico Approach Triterpene Glycoside of H. atra Targeting Orotidine 5-Monophosphate Decarboxylase Protein (PfOMPDC) in P. falciparum Infection Mechanism. [PDF]

Biomed Res Int
This study accessed the potential antimalarial activity of triterpene glycoside of H. atra through targeting orotidine 5‐monophosphate decarboxylase protein (PfOMPDC) in P. falciparum by molecular docking. Nine triterpene glycosides from H. atra extract modeled the structure by the Corina web server and interacted with PfOMPDC protein by using Hex 8.0 ...
Utami PD, Setianingsih H, Sari DRT.
europepmc   +2 more sources

CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica

Metabolic Engineering Communications, 2020
CRISPR-Cas9 has been widely adopted as the basic toolkit for precise genome-editing and engineering in various organisms. Alternative to Cas9, Cas12 or Cpf1 uses a simple crRNA as a guide and expands the protospacer adjacent motif (PAM) sequence to TTTN.
Zhiliang Yang, Harley Edwards, Peng Xu
doaj   +1 more source

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Microbial Biotechnology, 2020
Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species.
Ke Liu, Bin Sun, Hao You, Jun‐Liang Tu, Xuya Yu, Peng Zhao, Jun‐Wei Xu   +6 more
doaj   +1 more source

Activity of Yeast Orotidine-5′-phosphate Decarboxylase in the Absence of Metals [PDF]

Journal of Biological Chemistry, 1999
Yeast orotidine-5'-phosphate decarboxylase was recently shown to contain zinc and to be inhibited by zinc-complexing agents. When the gene for the yeast enzyme was expressed in Escherichia coli, the gene product was devoid of metal atoms but exhibited a specific activity and molecular mass similar to those of the enzyme obtained directly from yeast ...
B G, Miller, J A, Smiley, S A, Short, R, Wolfenden   +3 more
openaire   +2 more sources

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

BMC Microbiology, 2020
Background ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure.
Jinxin Zheng, Yang Wu, Zhiwei Lin, Guangfu Wang, Sibo Jiang, Xiang Sun, Haopeng Tu, Zhijian Yu, Di Qu   +8 more
doaj   +1 more source

Coupling between Catalytic Loop Motions and Enzyme Global Dynamics [PDF]

, 2012
Catalytic loop motions facilitate substrate recognition and binding in many enzymes. While these motions appear to be highly flexible, their functional significance suggests that structure-encoded preferences may play a role in selecting particular ...
Ahmet Bakan, Dennis R. Livesay, Duygu Kocaman, Ivet Bahar, Pemra Doruker, Zeynep Kurkcuoglu   +5 more
core   +4 more sources

Activity‐Based Protein Profiling in Methicillin‐Resistant Staphylococcus aureus Reveals the Broad Reactivity of a Carmofur‐Derived Probe

ChemBioChem, Volume 24, Issue 21, November 2, 2023., 2023
Enzyme targets of activity‐based probes built on the anti‐neoplastic drug and antimicrobial agent carmofur in methicillin‐resistant Staphylococcus aureus (MRSA) have been identified. Chemoproteomic profiling revealed a broad reactivity of the probe against both cysteine and serine hydrolases, and identified uncharacterized bacterial enzymes; this has ...
Md Jalal Uddin, Hermen S. Overkleeft, Christian S. Lentz   +2 more
wiley   +1 more source

Exploring Aspartate Transcarbamoylase: A Promising Broad-Spectrum Target for Drug Development. [PDF]

Chembiochem
Pyrimidine biosynthesis is a pivotal process shared by both eukaryotes and prokaryotes. This review examines the applications and advantages of a series of inhibitors specifically designed to target aspartate transcarbamylase within the de novo biosynthetic pathway of the malarial parasite.
Chen S, Mondile Q, Du X, Wang C, Mukim M, Wrenger C, Dömling ASS, Tastan Bishop Ö, Groves MR.   +8 more
europepmc   +2 more sources

Physiologic Medium Rewires Cellular Metabolism and Reveals Uric Acid as an Endogenous Inhibitor of UMP Synthase [PDF]

, 2018
A complex interplay of environmental factors impacts the metabolism of human cells, but neither traditional culture media nor mouse plasma mimic the metabolite composition of human plasma.
Abu-Remaileh, Monther, Cantor, Jason R., Freinkman, Elizaveta, Gao, Xin, Kanarek, Naama, Lewis, Caroline A., Louissaint, Abner, Sabatini, David   +7 more
core   +1 more source