Results 21 to 30 of about 4,091 (157)

Cloning and Overexpressing Membrane Proteins Using Pichia pastoris (Komagataella phaffii)

Current Protocols, Volume 3, Issue 11, November 2023., 2023
Abstract Understanding the structure and function of key proteins located within biological membranes is essential for fundamental knowledge and therapeutic applications. Robust cell systems allowing their actual overexpression are required, among which stands the methylotrophic yeast Pichia pastoris.
Magali Schwob, Valérie Kugler, Renaud Wagner   +2 more
wiley   +1 more source

Dissecting a Charged Network at the Active Site of Orotidine-5′-phosphate Decarboxylase [PDF]

Journal of Biological Chemistry, 2001
The crystal structure of yeast orotidine-5'-phosphate decarboxylase in complex with the postulated transition state analog, 6-hydroxyuridine-5'-phosphate, reveals contacts between this inhibitor and a novel quartet of charged residues (Lys-59, Asp-91, Lys-93, and Asp-96) within the active site. The structure also suggests a possible interaction between
B G, Miller, M J, Snider, R, Wolfenden, S A, Short   +3 more
openaire   +2 more sources

Integrated genomics and phenotype microarray analysis of Saccharomyces cerevisiae industrial strains for rice wine fermentation and recombinant protein production

Microbial Biotechnology, Volume 16, Issue 11, Page 2161-2180, November 2023., 2023
The integrated genomics and phenotype microarray analysis of industrial S. cerevisiae strains elucidated their evolutionary history and genomic elements responsible for metabolic and physiological characteristics associated with biotechnical applications.
Ye Ji Son, Min‐Seung Jeon, Hye Yun Moon, Jiwon Kang, Da Min Jeong, Dong Wook Lee, Jae Ho Kim, Jae Yun Lim, Jeong‐Ah Seo, Jae‐Hyung Jin, Yong‐Sun Bahn, Seong‐il Eyun, Hyun Ah Kang   +12 more
wiley   +1 more source

Regulation of virulence in Francisella tularensis by small non-coding RNAs [PDF]

, 2011
Using a cDNA cloning and sequencing approach we have shown that Francisella tularensis expresses homologues of several small RNAs (sRNAs) that are well-conserved among diverse bacteria.
Alain Charbit, Eric Frapy, Guillaume Postic, Iharilalao Dubail, Jonathan Livny, Karin L. Meibom, Marion Dupuis   +6 more
core   +2 more sources

Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic Mucorales Mucor circinelloides

G3: Genes, Genomes, Genetics, 2017
Mucor circinelloides is a human pathogen, biofuel producer, and model system that belongs to a basal fungal lineage; however, the genetics of this fungus are limited.
Alexis Garcia, Gloria Adedoyin, Joseph Heitman, Soo Chan Lee   +3 more
doaj   +1 more source

Host reticulocytes provide metabolic reservoirs that can be exploited by malaria parasites [PDF]

, 2015
Human malaria parasites proliferate in different erythroid cell types during infection. Whilst Plasmodium vivax exhibits a strong preference for immature reticulocytes, the more pathogenic P. falciparum primarily infects mature erythrocytes.
Barrett, Michael P., Creek, Darren J., De Souza, David, Evans, Krystal J., McConville, Malcolm J., Muller, Sylke, Schofield, Louis, Srivastava, Anubhav, Waters, Andrew P.   +8 more
core   +1 more source

Structure and mechanism of acetolactate decarboxylase [PDF]

, 2013
Acetolactate decarboxylase catalyzes the conversion of both enantiomers of acetolactate to the (R)-enantiomer of acetoin, via a mechanism that has been shown to involve a prior rearrangement of the non-natural (R)-enantiomer substrate to the natural (S ...
Anand R., Chan K. K., Collaborative Computational Project Number 4, Crout D. H. G., Crout D. H. G., Crout D. H. G., Crout D. H. G., Crout D. H. G., Dean Rea, DeLano W. L., Dolin M. I., Drake A. F., Dulieu C., Fisher Z., Fusetti F., Gerlt J. A., Hall D. R., Hill R. K., Ho M.-C., Jones T. A., Kabsch W., Løken J. P, Martin Wills, Moen A. R., Murshudov G. N., Najmudin S., Okrasa K., Perrakis A., Read R. J., Robins D. J., Shabir Najmudin, Stritzke K., Størmer F. C., Victoria A. Marlow, Vilmos Fülöp, Vinogradov M., Yan Y.   +36 more
core   +1 more source

Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit

Angewandte Chemie, Volume 137, Issue 29, July 14, 2025.
A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo, Aman Ishaqat, Junlin Chen, Fabian Wiertz, Adrian Kuzmanović, Matthias Bartneck, Fabian Kiessling, Andreas Herrmann   +7 more
wiley   +2 more sources

New functional families (FunFams) in CATH to improve the mapping of conserved functional sites to 3D structures. [PDF]

, 2012
CATH version 3.5 (Class, Architecture, Topology, Homology, available at http://www.cathdb.info/) contains 173 536 domains, 2626 homologous superfamilies and 1313 fold groups.
Cuff, Alison L, Dawson, Natalie L, Dessailly, Benoit H, Furnham, Nicholas, Lee, David, Lees, Jonathan G, Lewis, Tony E, Orengo, Christine A, Rentzsch, Robert, Sillitoe, Ian, Studer, Romain A, Thornton, Janet M, Yeats, Corin   +12 more
core   +1 more source

An Updated genome annotation for the model marine bacterium Ruegeria pomeroyi DSS-3 [PDF]

, 2014
When the genome of Ruegeria pomeroyi DSS-3 was published in 2004, it represented the first sequence from a heterotrophic marine bacterium. Over the last ten years, the strain has become a valuable model for understanding the cycling of sulfur and carbon ...
Adam R Rivers, Christa B Smith, Mary Moran   +2 more
core   +8 more sources