Results 21 to 30 of about 1,806 (131)

CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica

Metabolic Engineering Communications, 2020
CRISPR-Cas9 has been widely adopted as the basic toolkit for precise genome-editing and engineering in various organisms. Alternative to Cas9, Cas12 or Cpf1 uses a simple crRNA as a guide and expands the protospacer adjacent motif (PAM) sequence to TTTN.
Zhiliang Yang, Harley Edwards, Peng Xu
doaj  

Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit

Angewandte Chemie International Edition, EarlyView.
A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo, Aman Ishaqat, Junlin Chen, Fabian Wiertz, Adrian Kuzmanović, Matthias Bartneck, Fabian Kiessling, Andreas Herrmann   +7 more
wiley   +1 more source

Improvement of β‐Xylosidase and Endoxylanase Activities in Talaromyces amestolkiae by Genetic Manipulation of the Transcriptional Activator XlnR

Microbial Biotechnology, Volume 18, Issue 5, May 2025.
A successful transformation procedure for the genetic manipulation of the fungus Talaromyces amestolkiae was developed, enabling the forced expression of the transcriptional activator XlnR. A mutant version of XlnR carrying a Val785Phe substitution led to remarkably higher β‐xylosidase and endoxylanase activities compared to the wild‐type strain, which
Ana Pozo‐Rodríguez, Miguel Ángel Peñalva, Jorge Barriuso, Eduardo A. Espeso, María Jesús Martínez   +4 more
wiley   +1 more source

Metabolism and signaling crosstalk in glioblastoma progression and therapy resistance

Molecular Oncology, Volume 19, Issue 3, Page 592-613, March 2025.
Glioblastoma is one of the most lethal human cancers with no effective treatment nowadays. Here, we review the increasing amount of evidence supporting the connection between cell signaling deregulation and metabolic reprogramming as a key event in glioblastoma resistance to cancer therapy.
Laura Zarzuela, Raúl V. Durán, Mercedes Tomé   +2 more
wiley   +1 more source

The cis‐prenyltransferase protein family in Taraxacum koksaghyz

The Plant Journal, Volume 121, Issue 3, February 2025.
Significance Statement The novelty of this work lies in the comprehensive description of cis‐prenyl transferases in the rubber‐producing plant Taraxacum koksaghyz. This provides valuable insights for breeding and rational protein design in heterologous systems.
Boje Müller, Eva Niephaus, Wolfgang Eisenreich, Jan Niklas Bröker, Richard M. Twyman, Dirk Prüfer, Christian Schulze Gronover   +6 more
wiley   +1 more source

Mutualistic interactions of lactate‐producing lactobacilli and lactate‐utilizing Veillonella dispar: Lactate and glutamate cross‐feeding for the enhanced growth and short‐chain fatty acid production

Microbial Biotechnology, Volume 17, Issue 5, May 2024.
The study investigates the symbiotic interaction between Veillonella dispar and three lactic acid bacteria strains, revealing a robust mutualism primarily with Lactobacillus acidophilus. Through co‐culture experiments and transcriptomic analysis, the research elucidates a complex metabolic relationship involving cross‐feeding of lactate and glutamate ...
Shi‐Min Zhang, Jia‐He Hung, Tran Ngoc Yen, Shir‐Ly Huang   +3 more
wiley   +1 more source

Safety and efficacy of a feed additive consisting of 6‐phytase produced by Aspergillus oryzae DSM 33737 (HiPhorius™) for all poultry, all Suidae and all fin fish (DSM Nutritional Products Ltd)

EFSA Journal, Volume 22, Issue 3, March 2024.
Abstract Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of 6‐phytase produced by the genetically modified strain Aspergillus oryzae DSM 33737 (HiPhorius™ 10, 40, 20L and 50L) as a zootechnical feed ...
EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Birgit Dusemund, Mojca Durjava, Maryline Kouba, Marta López‐Alonso, Secundino López Puente, Francesca Marcon, Baltasar Mayo, Alena Pechová, Mariana Petkova, Fernando Ramos, Roberto Edoardo Villa, Ruud Woutersen, Noël Dierick, Henriqueta Louro, Giovanna Martelli, Luca Tosti, Montserrat Anguita, Joana P. Firmino, Matteo L. Innocenti, Elisa Petenatti, Fabiola Pizzo, Jordi Ortuño   +26 more
wiley   +1 more source

Improving homology‐directed repair by small molecule agents for genetic engineering in unconventional yeast?—Learning from the engineering of mammalian systems

Microbial Biotechnology, Volume 17, Issue 2, February 2024.
Here we consolidate knowledge on chemical agents that affect DNA repair meechanisms and how they have been employed to enhance sucess in mammalian cells and some yeast. Abstract The ability to precisely edit genomes by deleting or adding genetic information enables the study of biological functions and the building of efficient cell factories.
Min Lu, Sonja Billerbeck
wiley   +1 more source

Molecular Regulation of Histamine Synthesis [PDF]

arXiv, 2018
Histamine is a critical mediator of IgE/ cell-mediated anaphylaxis, a neurotransmitter and a regulator of gastric acid secretion. Histamine is a monoamine synthesized from the amino acid histidine through a reaction catalyzed by the enzyme histidine decarboxylase (HDC), which removes carboxyl group from histidine.
arxiv  

Dissecting a Charged Network at the Active Site of Orotidine-5′-phosphate Decarboxylase [PDF]

, 2001
Brian G. Miller, Mark J. Snider, Richard Wolfenden, Steven A. Short   +3 more
openalex   +3 more sources