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Folding of outer membrane proteins
Archives of Biochemistry and Biophysics, 2013Outer membrane proteins (OMPs) represent a large group of β-barrel proteins found both in the membranes of both bacteria and eukaryotes. Their general ease of expression and refolding and straightforward methods to monitor their degree of folding conspire to make OMPs excellent model systems to investigate how the membrane environment and other ...
Otzen, Daniel, Andersen, Kell K
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Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine
Vaccine, 2020The Chlamydial outer membrane complex (COMC) from the elementary body (EB) is a protein rich insoluble outer membrane shell from which cytosolic proteins have been extracted with detergent. In this study we conducted mass spectrometry experiments to detect proteins in the COMC prepared from C. muridarum EB.
Hong, Yu +7 more
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Biogenesis of Outer Membrane Proteins
2003The cell envelope of Gram-negative bacteria consists of two membranes, the inner membrane, which is a phospholipid bilayer, and the outer membrane, which is an asymmetrical bilayer with phospholipids and lipopolysaccharides (LPS) in the inner and outer monolayers, respectively. The membranes are separated by the peptidoglycan-containing periplasm. Both
Tommassen, J.P.M., Voulhoux, J.R.
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Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles
The Journal of Microbiology, 2012Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of ...
Dong Chan, Moon +7 more
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Outer-Membrane Permeability of Bacteria
CRC Critical Reviews in Microbiology, 1986Gram-negative bacteria evolved to survive under the conditions in which a number of hazardous compounds are abundant. The outer membrane which protects the cell interior acts as a barrier against such hazardous agents, yet the cells must incorporate the chemicals that are essential for the cellular activity.
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Assembly of Bacterial Outer Membrane Proteins
2012Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane proteins (OMPs). Here, we describe three biochemical methods that can be used in such studies to evaluate the proper assembly of OMPs into the ...
Grijpstra, J. +2 more
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Prokaryotes: The Outer Membrane
2000Many prokaryotic species have a membrane outside the plasma membrane (Fig. 5.1). They are traditionally called Gram-negative with reference to the fact that they do not retain a particular blue dye used for the observation at the optical microscope. This name should be abandoned for two reasons. First, because it recalls a person.
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