Isolation of the outer membrane and characterization of the major outer membrane protein from Spirochaeta aurantia [PDF]
Journal of Bacteriology, 1987 The outer membrane of Spirochaeta aurantia was isolated after cells were extracted with sodium lauryl sarcosinate and was subsequently purified by differential centrifugation and KBr isopycnic gradient centrifugation. The purified outer membrane was obtained in the form of carotenoid-containing vesicles.
A M, Kropinski +5 more
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Phosphoproteins and protein-kinase activity in isolated envelopes of pea (Pisum sativum L.) chloroplasts [PDF]
, 1985 A protein kinase was found in envelope membranes of purified pea (Pisum sativum L.) chloroplasts. Separation of the two envelope membranes showed that most of the enzyme activity was localized in the outer envelope.
Soll, Jürgen
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Functional Versatility of Vibrio cholerae Outer Membrane Proteins
Applied MicrobiologyA key feature that differentiates Gram-positive and Gram-negative bacteria is the outer membrane, an asymmetric membrane composed of lipopolysaccharides, phospholipids, lipoproteins and integral proteins, including the outer-membrane proteins (OMPs).
Annabelle Mathieu-Denoncourt +1 more
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BMC Genomics, 2009 Background Chlamydiae are obligate intracellular bacteria comprising some of the most important bacterial pathogens of animals and humans. Although chlamydial outer membrane proteins play a key role for attachment to and entry into host cells, only few ...
Myers Garry +5 more
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Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris [PDF]
, 2008 Sidhu VK, Vorhölter F-J, Niehaus K, Watt SA. Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology.
Steven A Watt +11 more
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Outer envelope membranes from chloroplasts are isolated as right-side-out vesicles [PDF]
, 1992 Outer envelope membranes were isolated from purified chloroplasts of pea leaves. The sidedness of the vesicles was analyzed by (i) aqueous polymer-two phase partitioning, (ii) the effect of limited proteolysis on the outer-envelope proteins (OEP) 86 and ...
Eichacker, Susanne +2 more
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Outer membrane protein folding from an energy landscape perspective
BMC Biology, 2017 The cell envelope is essential for the survival of Gram-negative bacteria. This specialised membrane is densely packed with outer membrane proteins (OMPs), which perform a variety of functions.
Bob Schiffrin +2 more
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A crucial role of the mitochondrial protein import receptor MOM19 for the biogenesis of mitochondria [PDF]
, 1994 The novel genetic method of "sheltered RIP" (repeat induced point mutation) was used to generate a Neurospora crassa mutant in which MOM19, a component of the protein import machinery of the mitochondrial outer membrane, can be depleted.
W Neupert +11 more
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Structural insight into mitochondrial β-barrel outer membrane protein biogenesis
Nature Communications, 2020 The Sorting and Assembly Machinery (SAM) complex folds beta-barrel proteins and inserts them into the mitochondrial outer membrane. Here authors report cryoEM structures of the SAM complex from Myceliophthora thermophila, which reveals a GST-like fold ...
Kathryn A. Diederichs +8 more
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The structure of bacterial outer membrane proteins
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2002 Integral membrane proteins come in two types, alpha-helical and beta-barrel proteins. In both types, all hydrogen bonding donors and acceptors of the polypeptide backbone are completely compensated and buried while nonpolar side chains point to the membrane. The alpha-helical type is more abundant and occurs in cytoplasmic (or inner) membranes, whereas
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