Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Cleaner Engineering and TechnologyIntegrating sustainability considerations into equipment-level performance measurement remains a methodological challenge in manufacturing systems. While MES/SCADA platforms enable high-frequency operational monitoring, conventional Overall Equipment ...
Supatchaya Chotayakul +1 more
doaj +1 more source
Lessons Learned and Next Steps in Energy Efficiency Measurement and Attribution: Energy Savings, Net to Gross, Non-Energy Benefits, and Persistence of Energy Efficiency Behavior [PDF]
, 2009 This white paper examines four topics addressing evaluation, measurement, and attribution of direct and indirect effects to energy efficiency and behavioral programs: Estimates of program savings (gross); Net savings derivation through free ridership ...
Colby, Jane +2 more
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FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Solar photochemical process engineering for production of fuels and chemicals [PDF]
The engineering costs and performance of a nominal 25,000 scmd (883,000 scfd) photochemical plant to produce dihydrogen from water were studied. Two systems were considered, one based on flat-plate collector/reactors and the other on linear parabolic ...
Biddle, J. R. +2 more
core +1 more source
Integrating Smart Ceiling Fans and Communicating Thermostats to Provide Energy-Efficient Comfort [PDF]
, 2020 The project goal was to identify and test the integration of smart ceiling fans and communicating thermostats. These highly efficient ceiling fans use as much power as an LED light bulb and have onboard temperature and occupancy sensors for automatic ...
Arens, Edward +12 more
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
End region and current consolidation effects upon the performance of an MHD channel for the ETF conceptual design [PDF]
The effects of MHD channel end regions on the overall power generation were considered. The peak plant thermodynamic efficiency was found to be slightly lower than for the active region (41%).
Smith, J. M., Wang, S. Y.
core +1 more source
Scenarios of energy efficiency and CO2 emissions reduction potential in the buildings sector in China to year 2050 [PDF]
, 2018 As China’s rapid urbanization continues and urban dwellers become more affluent, energy use in buildings is expected to grow. To understand how this growth can be slowed, we explore four scenarios for Chinese buildings, ranging from a high-energy-demand ...
Feng, W +4 more
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