Results 61 to 70 of about 202,319 (254)

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Application of The Overall Equipment Effectiveness (OEE) Method in Increasing The Efficiency Of Mangel / Crepper Machine

open access: yesJournal of Engineering Science and Technology Management (JES-TM), 2021
Riau Crumb Rubber Factory (PT. RICRY) is a manufacturing company engaged in processing raw rubber into semi-finished goods. The raw materials used are Ojol and Lump which come from rubber farmers in the Riau area themselves. The products produced consist of two types based on their quality, namely SIR 10 and SIR 20 which will be reprocessed as raw ...
openaire   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

WAYS TO IMPROVE THE EFFICIENCY OF TECHNOLOGICAL EQUIPMENT

open access: yesШәкәрім университетінің хабаршысы: Техника ғылымдар
Increasing the productivity of production systems and equipment in the meat industry is inextricably linked with maintenance activities. The article analyzes the influence of manufacturability, hygiene, reliability, simplicity of equipment design on the ...
G. Abdilova   +4 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

The Role of Total Productivity Maintenance In Improving The Overall Equipment Efficiency: A Case study

open access: yes, 2021
The study's purpose is to analyze the working efficiency of the machines and determine the proposed improvement. This study was conducted using four steps, namely, determining the level of machine effectiveness , determining the value of losses affecting the low level of effectiveness, using Pareto schemes to determine the most widespread losses ...
openaire   +2 more sources

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

open access: yesFEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Analysis of influencing factors on the man-machine system efficiency in high-altitude mines [PDF]

open access: yesE3S Web of Conferences
High-altitude areas contain a large amount of high-quality mineral resources in China, and the development of mineral resources in these areas has made significant contributions to social and economic development.
Guo Duiming   +3 more
doaj   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

open access: yesFEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li   +7 more
wiley   +1 more source

Research on new systems engineering technologies for aviation equipment R&D overall design

open access: yesXibei Gongye Daxue Xuebao
R&D overall of equipment determines the selection of technical solutions, the design of developing routes, and the generation of combat capabilities, which is a systematic engineering of overall and decision-making significance.
LI Miao, YANG Shuifeng, ZHANG Yizhuo
doaj   +1 more source

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