The Oxidosqualene Cyclase from the Oomycete Saprolegnia Parasitica Synthesizes Lanosterol as a Single Product [PDF]
Frontiers in Microbiology, 2016 The first committed step of sterol biosynthesis is the cyclisation of 2,3-oxidosqualene to form either lanosterol or cycloartenol. This is catalyzed by an oxidosqualene cyclase.
Paul Dahlin +5 more
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Characterization of the channel constriction allowing the access of the substrate to the active site of yeast oxidosqualene cyclase. [PDF]
PLoS ONE, 2011 In oxidosqualene cyclases (OSCs), an enzyme which has been extensively studied as a target for hypocholesterolemic or antifungal drugs, a lipophilic channel connects the surface of the protein with the active site cavity.
Simonetta Oliaro-Bosso +5 more
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Site-directed mutagenesis identified the key active site residues of 2,3-oxidosqualene cyclase HcOSC6 responsible for cucurbitacins biosynthesis in Hemsleya chinensis [PDF]
Frontiers in Plant Science, 2023 Hemsleya chinensis is a Chinese traditional medicinal plant, containing cucurbitacin IIa (CuIIa) and cucurbitacin IIb (CuIIb), both of which have a wide range of pharmacological effects, including antiallergic, anti-inflammatory, and anticancer ...
Xia Li +20 more
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Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids [PDF]
Synthetic and Systems Biotechnology, 2022 Zea mays (maize) is an important agricultural crop that produces a variety of valuable terpenoids, including several triterpenoids. However, no oxidosqualene cyclase (OSC) enzymes, which catalyze the first step in triterpenoid biosynthesis, have been ...
Zhenjun Fan +5 more
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The triterpenoid saponin content difference is associated with the two type oxidosqualene cyclase gene copy numbers of Pulsatilla chinensis and Pulsatilla cernua [PDF]
Frontiers in Plant Science, 2023 Pulsatilla chinensis is an important medicinal herb, its dried radix is used to treat the inflammation since ancient China. Triterpenoid saponins are proved to be the main active compounds of Pulsatilla genus.
Xianju Liu +4 more
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Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial. [PDF]
Cell Chem Biol, 2021 Phenotypic screening identified a benzothiophene compound with activity against Leishmania donovani, the causative agent of visceral leishmaniasis. Using multiple orthogonal approaches, oxidosqualene cyclase (OSC), a key enzyme of sterol biosynthesis ...
Paradela LS +14 more
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Oxidosqualene Cyclase Knock-Down in Latex of Taraxacum koksaghyz Reduces Triterpenes in Roots and Separated Natural Rubber [PDF]
Molecules, 2019 In addition to natural rubber (NR), several triterpenes are synthesized in laticifers of the Russian dandelion (Taraxacum koksaghyz). Detailed analysis of NR and resin contents revealed different concentrations of various pentacyclic triterpenes such as &
Nicole van Deenen +3 more
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Genome-Wide Analysis of Oxidosqualene Cyclase Genes in Artemisia annua: Evolution, Expression, and Potential Roles in Triterpenoid Biosynthesis [PDF]
Current Issues in Molecular BiologyPlant triterpenoids are structurally diverse specialized metabolites with significant ecological, medicinal, and agricultural importance. Oxidosqualene cyclases (OSCs) catalyze the crucial cyclization step in triterpenoid biosynthesis, generating the ...
Changfeng Guo, Si Xu, Xiaoyun Guo
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Metabolic engineering of Phaeodactylum tricornutum for heterologous production of the plant triterpenoid friedelin [PDF]
Microbial Cell FactoriesBackground Friedelin is a pharmacologically valuable pentacyclic triterpenoid with anti-inflammatory, anticancer, antiviral, and antiobesity properties. Conventional methods of friedelin production rely on solvent-intensive extraction from plant biomass,
Khanh Nguyen +4 more
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Should oxidosqualene cyclase in the cholesterol biosynthetic pathway be considered an anti-cancer target? [PDF]
Frontiers in Cell and Developmental Biology, 2022 Slight SH, Hyder SM
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