Results 31 to 40 of about 5,693 (184)

Differential transcriptome analysis of glandular and filamentous trichomes in Artemisia annua [PDF]

, 2013
Background: The medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be
Deforce, Dieter, Goossens, Alain, Head, Steven R, Soetaert, Sandra, Van Neste, Christophe, Van Nieuwerburgh, Filip, Vandewoestyne, Mado   +6 more
core   +4 more sources

A conserved amino acid residue critical for product and substrate specificity in plant triterpene synthases [PDF]

, 2016
Triterpenes are structurally complex plant natural products with numerous medicinal applications. They are synthesized through an origami-like process that involves cyclization of the linear 30 carbon precursor 2,3-oxidosqualene into different triterpene
Al-Qurainy, Andrew M. Hemmings, Anne Osbourn, Kushiro, Melissa Salmon, Paul E. O’Maille, Rachel E. Melton, Racolta, Ramesha B. Thimmappa, Richard K. Hughes, Robert E. Minto, Wiederstein, Wu   +12 more
core   +1 more source

A Soluble 2,3-Oxidosqualene Sterol Cyclase

Journal of Biological Chemistry, 1967
An enzyme catalyzing the anaerobic cyclization of 2,3-oxidosqualene into lanosterol has been extracted from hog liver microsomes by treatment with deoxycholate. Removal of deoxycholate, delipidation with ether, and ammonium sulfate precipitation yield a soluble 20-fold purified cyclase. No coenzyme requirements could be demonstrated for the cyclization
P D, Dean, P R, Ortiz de Montellano, K, Bloch, E J, Corey   +3 more
openaire   +2 more sources

Oxidosqualene Cyclase (OSC) Inhibitors for the Treatment of Dyslipidemia

CHIMIA, 2005
Novel inhibitors of oxidosqualene cyclase (OSC) for the treatment of dyslipidemia are reported. Starting point for the chemistry program was a set of compounds derived from a fungicide project which, in addition to high affinity for OSC from ...
Henrietta Dehmlow, Ralf Thoma, Armin Ruf, Narendra Panday, Elisabeth Von Der Mark, Olivier Morand, Hans Peter Märki, Manfred Kansy, Peter Hartman, Philippe Coassolo, Alexander Chucholowski, Denise Blum-Kaelin, Johannes Aebi, Jean Ackermann, Tanja Schulz-Gasch   +14 more
doaj   +1 more source

The TriForC database : a comprehensive up-to-date resource of plant triterpene biosynthesis [PDF]

, 2018
Triterpenes constitute a large and important class of plant natural products with diverse structures and functions. Their biological roles range from membrane structural components over plant hormones to specialized plant defence compounds.
Bak, Soren, Botzki, Alexander, Coppens, Frederik, De Bo, Christof, Goossens, Alain, Iñigo, Sabrina, Kreft, Lukasz, Miettinen, Karel, Pollier, Jacob   +8 more
core   +2 more sources

Inactivation and activation of various membranal enzymes of the cholesterol biosynthetic pathway by digitonin.

Journal of Lipid Research, 1989
The activity of rat liver microsomal squalene epoxidase is inhibited effectively by digitonin. Concentrations of 0.8 to 1.2 mg/ml of digitonin cause total inhibition of microsomal (0.75 mg protein/ml) squalene epoxidase either in microsomes that were ...
H Eilenberg, E Klinger, F Przedecki, I Shechter   +3 more
doaj   +1 more source

Integrated Metabolomic and Transcriptomic Analysis and Identification of Dammarenediol-II Synthase Involved in Saponin Biosynthesis in Gynostemma longipes

Frontiers in Plant Science, 2022
Gynostemma longipes contains an abundance of dammarane-type ginsenosides and gypenosides that exhibit extensive pharmacological activities. Increasing attention has been paid to the elucidation of cytochrome P450 monooxygenases (CYPs) and UDP-dependent ...
Shuang Ye, Lei Feng, Shiyu Zhang, Shiyu Zhang, Yingchun Lu, Guisheng Xiang, Bo Nian, Qian Wang, Shuangyan Zhang, Wanling Song, Ling Yang, Xiangyu Liu, Baowen Feng, Guanghui Zhang, Bing Hao, Shengchao Yang   +15 more
doaj   +1 more source

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

Microbial Cell Factories, 2020
Background Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking ...
Nikolaj L. Hansen, Karel Miettinen, Yong Zhao, Codruta Ignea, Aggeliki Andreadelli, Morten H. Raadam, Antonios M. Makris, Birger L. Møller, Dan Stærk, Søren Bak, Sotirios C. Kampranis   +10 more
doaj   +1 more source

Effects of specific inhibition of sterol biosynthesis on the uptake and utilization of low density lipoprotein cholesterol by HepG2 cells.

Journal of Lipid Research, 1991
Treatment of HepG2 cells in lipoprotein-deficient media with 4,4,10 beta-trimethyl-trans-decal-3 beta-ol (TMD) abolished the incorporation of [3H]acetate into cholesterol with concomitant accumulation of squalene 2,3(S)-oxide and squalene 2,3(S):22(S),23-
SR Panini, GT Everson, TA Spencer
doaj   +1 more source

Identification of oxidosqualene cyclases associated with saponin biosynthesis from Astragalus membranaceus reveals a conserved motif important for catalytic function

Journal of Advanced Research, 2023
Introduction: Triterpenoids and saponins have a broad range of pharmacological activities. Unlike most legumes which contain mainly oleanane-type scaffold, Astragalus membranaceus contains not only oleanane-type but also cycloartane-type saponins, for ...
Kuan Chen, Meng Zhang, Lulu Xu, Yang Yi, Linlin Wang, Haotian Wang, Zilong Wang, Jiangtao Xing, Pi Li, Xiaohui Zhang, Xiaomeng Shi, Min Ye, Anne Osbourn, Xue Qiao   +13 more
doaj   +1 more source