建立一种非同位素的不对称聚合酶链反应-单链构象多态性技术(aPCR-SSCP):通过不对称PCR(aPCR)获得单链,普通PAGE电泳分离,经银染快速准确检出突变;应用这种方法,研究了4株鼻咽癌细胞株CNE1,CNE2,HK1和SUNE1中肿瘤抑制基因P53基因突变,证实CNE1,CNE2在第8外显子,HK1在第5外显子有突变,并发现新建立的细胞株SUNE1存在第8外显子的突变。
doaj
[Expression Characteristics and Prognostic Study of PPP1R13L in Brain Metastases of Lung Adenocarcinoma]. [PDF]
Liu L, Fan P, Chang C, Wang R.
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[Effects of miR-100-5p on proliferation and apoptosis of acute myeloid leukemia cells and the underlying molecular mechanism]. [PDF]
Fan XR, Yang Y, Sun Y, DU CM.
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[Advances in basic and experimental diagnostic research on liver diseases in 2025]. [PDF]
Chen DB, Yang RF, Chen HS.
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[Bioinformatics screening and analysis of key genes of ferroptosis and autophagy in alcoholic liver disease and their validation]. [PDF]
Zhang QQ +4 more
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目的应用生物信息学的方法探讨趋化素样因子超家族成员(chemokine-like factor-like MARVEL transmembrane domain containing member/chemokine-like factor superfamily member,CMTM/CKLFSF,CMTM)在胰腺癌中异常表达的临床病理特征,并分析相关基因共表达的意义。方法通过GEPIA2、HPA和Kaplan-Meier plotter数据库分析CMTM家族成员在胰腺癌中的表达和预后价值 ...
黄冠宁 +5 more
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[Advances on efficacy and mechanisms of acid fibroblast growth factor for promoting injured tissue repair]. [PDF]
Zhu F +5 more
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[PINK1 suppresses colorectal cancer cell growth through epigenetic regulation of histone modifications]. [PDF]
Wang M, Luan S, Fan X, Han D, Zhu Y.
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[CAR-T cell therapy bridging to allogeneic hematopoietic stem cell transplantation for diffuse large B-cell Richter syndrome: a case report and literature review]. [PDF]
Wang Y +5 more
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[Acupuncture alleviates chronic airway inflammation in obese asthmatic mice by downregulating Vnn1 and FAM126B]. [PDF]
Li X, Ye T, Xi L, Li C, Liu H.
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