Results 251 to 260 of about 48,352 (276)
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C&EN Global Enterprise, 2020
Faced with resistance from antitrust authorities concerned about reduced competition, Illumina has dropped plans to acquire its next-generation DNA-sequencing (NGS) rivalPacific Biosciences of Cali...
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Faced with resistance from antitrust authorities concerned about reduced competition, Illumina has dropped plans to acquire its next-generation DNA-sequencing (NGS) rivalPacific Biosciences of Cali...
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Structural variation discovery in wheat using PacBio high-fidelity sequencing
The Plant Journal, 2023AbstractBackgroundStructural variations (SVs) pervade plant genomes and contribute substantially to the phenotypic diversity. However, most SVs were ineffectively assayed because of their complex nature and the limitations of early genomic technologies.
Zhiliang Zhang +14 more
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PacBio for Haplotyping in Gene Families
2017The throughput and read length provided by Pacific Bioscience (PacBio) Single Molecule Real Time (SMRT) sequencing platform makes it feasible to construct contiguous, non-chimeric sequences. This is especially useful for genes with repetitive sequences in their gene bodies in gene families.
Wei, Zhang, Joachim, Messing
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2SNV: Quasispecies reconstruction from PacBio reads
2015 IEEE 5th International Conference on Computational Advances in Bio and Medical Sciences (ICCABS), 2015Pacific Biosciences (PacBio) sequencing is providing thousands of reads with the length up to 10,000 bases. In most cases this length is enough to cover entire region of interest however this technology has high (≈15%) error rate. We propose a method for viral haplotype reconstruction using long SMRT reads.
Alexander Artyomenko +5 more
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Inversion detection using PacBio long reads
2017 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 2017Structural variation is important in disease etiology and ecological adaptation. Prior work has focused on using either only short paired-end reads or a hybrid approach that combines long and short reads to detect structural variants. Few methods have focused solely on using long reads.
Shenglong Zhu +2 more
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Informatics for PacBio Long Reads
2019In this article, we review the development of a wide variety of bioinformatics software implementing state-of-the-art algorithms since the introduction of SMRT sequencing technology into the field. We focus on the three major categories of development: read mapping (aligning to reference genomes), de novo assembly, and detection of structural variants.
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Bifidobacterium Genome Assembly and Methylome Analysis Using Pacbio SMRT Sequencing
2021In this chapter, we present a generic method to achieve de novo genome assembly and methylome analysis of Bifidobacterium genomes using the Pacbio SMRT sequencing and SMRT Link pipeline. The methods described here cover the de novo Pacbio or hybrid Illumina-Pacbio assembly ideally intended to obtain a complete genome sequence, followed by the detection
Francesca, Bottacini +1 more
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US FTC challenges Illumina’s purchase of PacBio
C&EN Global Enterprise, 2020The US Federal Trade Commission has challenged Illumina’s planned $1.2 billion acquisition of Pacific Biosciences of California, its smaller next-generation DNA-sequencing (NGS) rival. The antitrust regulator says it has ordered an administrative trial on the deal for August and will seek a restraining order in federal court if necessary to prevent it ...
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Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII
2016Conventional mitochondrial-DNA (MT DNA) sequencing approaches use Sanger sequencing of 20-40 partially overlapping PCR fragments per individual, which is a time- and resource-consuming process. We have developed a high-throughput, accurate, fast, and cost-effective human MT DNA sequencing approach.
Rolf H A M, Vossen, Henk P J, Buermans
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