Application of third-generation sequencing to herbal genomics [PDF]
Frontiers in Plant Science, 2023 There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have
Longlong Gao +3 more
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Application of third-generation sequencing in cancer research [PDF]
Medical Review, 2021 In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
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Identification of rare thalassemia variants using third-generation sequencing
Frontiers in Genetics, 2023 Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu +11 more
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3GOLD: optimized Levenshtein distance for clustering third-generation sequencing data [PDF]
BMC Bioinformatics, 2022 Background Third-generation sequencing offers some advantages over next-generation sequencing predecessors, but with the caveat of harboring a much higher error rate. Clustering-related sequences is an essential task in modern biology.
Robert Logan +6 more
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Recovery of human gut microbiota genomes with third-generation sequencing [PDF]
Cell Death and Disease, 2021 Human gut microbiota modulates normal physiological functions, such as maintenance of barrier homeostasis and modulation of metabolism, as well as various chronic diseases including type 2 diabetes and gastrointestinal cancer. Despite decades of research,
Yanfei Li +8 more
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Benchmarking second and third-generation sequencing platforms for microbial metagenomics
Scientific Data, 2022 Measurement(s) mock community • Metagenomic profiling Technology Type(s) Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly Sample ...
Victoria Meslier +7 more
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Viral oncogenes, viruses, and cancer: a third-generation sequencing perspective on viral integration into the human genome [PDF]
Frontiers in Oncology, 2023 The link between viruses and cancer has intrigued scientists for decades. Certain viruses have been shown to be vital in the development of various cancers by integrating viral DNA into the host genome and activating viral oncogenes.
Ruichen Ye +9 more
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Analysis of rare thalassemia genetic variants based on third-generation sequencing [PDF]
Scientific Reports, 2022 Thalassemia is a group of common hereditary anemias that cause significant morbidity and mortality worldwide. However, precisely diagnosing thalassemia, especially rare thalassemia variants, is still challenging.
Cuiting Peng +11 more
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Application of third-generation sequencing technology for identifying rare α- and β-globin gene variants in a Southeast Chinese region [PDF]
BMC Medical GenomicsBackground Third-generation sequencing (TGS) based on long-read technology has been gradually used in identifying thalassemia and hemoglobin (Hb) variants.
Jianlong Zhuang +4 more
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Diagnostic value of third-generation nanopore sequencing in extrapulmonary tuberculosis
Frontiers in Cellular and Infection MicrobiologyBackgroundThis study aimed to explore the accuracy of third-generation nanopore sequencing to diagnose extrapulmonary tuberculosis (EPTB).MethodsSamples were collected from the lesions of 67 patients with suspected EPTB admitted between April 2022 and ...
Chang Song +11 more
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