Results 11 to 20 of about 1,856 (207)

Application of third-generation sequencing in cancer research [PDF]

Medical Review, 2021
In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
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Application of third-generation sequencing to herbal genomics

Frontiers in Plant Science, 2023
There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have
Longlong Gao, Wenjie Xu, Tianyi Xin, Jingyuan Song   +3 more
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Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
Syahzuwan Hassan, Rosnah Bahar, Muhammad Farid Johan, Ezzeddin Kamil Mohamed Hashim, Wan Zaidah Abdullah, Ezalia Esa, Faidatul Syazlin Abdul Hamid, Zefarina Zulkafli   +7 more
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Identification of rare thalassemia variants using third-generation sequencing

Frontiers in Genetics, 2023
Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu, Qianting Chen, Zonglei Zhang, Shiyi Peng, Jing Liu, Jialun Pang, Zhengjun Jia, Hui Xi, Jiaqi Li, Libao Chen, Yinyin Liu, Ying Peng   +11 more
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Recovery of human gut microbiota genomes with third-generation sequencing [PDF]

Cell Death and Disease, 2021
Human gut microbiota modulates normal physiological functions, such as maintenance of barrier homeostasis and modulation of metabolism, as well as various chronic diseases including type 2 diabetes and gastrointestinal cancer. Despite decades of research,
Yanfei Li, Yueling Jin, Jianming Zhang, Haoying Pan, Lan Wu, Dingsheng Liu, Jinlong Liu, Jing Hu, Junwei Shen   +8 more
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3GOLD: optimized Levenshtein distance for clustering third-generation sequencing data [PDF]

BMC Bioinformatics, 2022
Background Third-generation sequencing offers some advantages over next-generation sequencing predecessors, but with the caveat of harboring a much higher error rate. Clustering-related sequences is an essential task in modern biology.
Robert Logan, Zoe Fleischmann, Sofia Annis, Amy Wangsness Wehe, Jonathan L. Tilly, Dori C. Woods, Konstantin Khrapko   +6 more
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Benchmarking second and third-generation sequencing platforms for microbial metagenomics

Scientific Data, 2022
Measurement(s) mock community • Metagenomic profiling Technology Type(s) Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly Sample ...
Victoria Meslier, Benoit Quinquis, Kévin Da Silva, Florian Plaza Oñate, Nicolas Pons, Hugo Roume, Mircea Podar, Mathieu Almeida   +7 more
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Analysis of rare thalassemia genetic variants based on third-generation sequencing [PDF]

Scientific Reports, 2022
Thalassemia is a group of common hereditary anemias that cause significant morbidity and mortality worldwide. However, precisely diagnosing thalassemia, especially rare thalassemia variants, is still challenging.
Cuiting Peng, Haixia Zhang, Jun Ren, Han Chen, Ze Du, Tong Zhao, Aiping Mao, Ruofan Xu, Yulin Lu, He Wang, Xinlian Chen, Shanling Liu   +11 more
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Diagnostic value of third-generation nanopore sequencing in extrapulmonary tuberculosis

Frontiers in Cellular and Infection Microbiology
BackgroundThis study aimed to explore the accuracy of third-generation nanopore sequencing to diagnose extrapulmonary tuberculosis (EPTB).MethodsSamples were collected from the lesions of 67 patients with suspected EPTB admitted between April 2022 and ...
Chang Song, Chang Song, Chunyan Zhao, Chunyan Zhao, Yanrong Lin, Yingxing Nong, Aichun Huang, Shaoyong Xi, Xiaoying Wei, Chunmei Zeng, Shixiong Yang, Qingdong Zhu   +11 more
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An Overview of the Second and Third-Generation of DNA Sequencing Technologies [PDF]

Journal of Advanced Biomedical Sciences, 2017
DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid.
Fatemeh Ghorbani Parsa, Mohammad Ali Hossein Pour Feizi   +1 more
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