Results 11 to 20 of about 574,806 (288)
The Third-Generation Sequencing Challenge: Novel Insights for the Omic Sciences [PDF]
BiomoleculesThe understanding of the human genome has been greatly improved by the advent of next-generation sequencing technologies (NGS). Despite the undeniable advantages responsible for their widespread diffusion, these methods have some constraints, mainly ...
Carmela Scarano +5 more
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Third‐Generation Sequencing of Epigenetic DNA
Angewandte Chemie, 2023 AbstractThe discovery of epigenetic bases has revolutionised the understanding of disease and development. Among the most studied epigenetic marks are cytosines covalently modified at the 5 position. In order to gain insight into their biological significance, the ability to determine their spatiotemporal distribution within the genome is essential ...
Bethany Searle +3 more
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Diagnostics, 2023 Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
Syahzuwan Hassan +7 more
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A window into third generation sequencing [PDF]
Human Molecular Genetics, 2010 First- and second-generation sequencing technologies have led the way in revolutionizing the field of genomics and beyond, motivating an astonishing number of scientific advances, including enabling a more complete understanding of whole genome sequences and the information encoded therein, a more complete characterization of the methylome and ...
Eric E, Schadt +2 more
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Advances and Trends in Omics Technology Development
Frontiers in Medicine, 2022 The human history has witnessed the rapid development of technologies such as high-throughput sequencing and mass spectrometry that led to the concept of “omics” and methodological advancement in systematically interrogating a cellular system.
Xiaofeng Dai, Li Shen
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SequelTools: a suite of tools for working with PacBio Sequel raw sequence data
BMC Bioinformatics, 2020 Background PacBio sequencing is an incredibly valuable third-generation DNA sequencing method due to very long read lengths, ability to detect methylated bases, and its real-time sequencing methodology.
David E. Hufnagel +2 more
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Frontiers in Genetics, 2020 DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang +3 more
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Fertility & Reproduction, 2023 Background and Aims: In in vitro fertilization (IVF) cycles, some patients suffering with vaginosis showed poor reproductive outcome even transferring with good quality embryos.
Wei-Fang Chang +11 more
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Third generation indexing for third generation sequencing [PDF]
, 2020 AbstractIndexing of DNA sequences is the art of sorting massive genomic data in a user-friendly structure to enable rapid accessing and comparing of different patterns in the data. Current genome assemblers use general algorithms for string indexing that do not exploit the special structural arrangement of genomes.
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Jabba: hybrid error correction for long sequencing reads using maximal exact matches [PDF]
, 2015 Third generation sequencing platforms produce longer reads with higher error rates than second generation sequencing technologies. While the improved read length can provide useful information for downstream analysis, underlying algorithms are challenged
Audenaert, P. +4 more
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