Results 11 to 20 of about 6,398,546 (257)

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
Muhammad Farid Johan, Rosnah Bahar, Ezalia Esa, Faidatul Syazlin Abdul Hamid, Zefarina Zulkafli, Ezzeddin Kamil Mohamed Hashim, Syahzuwan Hassan, Wan Zaidah Abdullah, Abdullah, Wan Zaidah, Bahar, Rosnah, Mohamed Hashim, Ezzeddin Kamil, Johan, Muhammad Farid, Zulkafli, Zefarina, Esa, Ezalia, Abdul Hamid, Faidatul Syazlin, Hassan, Syahzuwan   +15 more
core   +2 more sources

Diagnostic value of third-generation nanopore sequencing in extrapulmonary tuberculosis [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundThis study aimed to explore the accuracy of third-generation nanopore sequencing to diagnose extrapulmonary tuberculosis (EPTB).MethodsSamples were collected from the lesions of 67 patients with suspected EPTB admitted between April 2022 and ...
Chang Song, Yingxing Nong, Chunyan Zhao, Aichun Huang, Xiaoying Wei, Yanrong Lin, Shaoyong Xi, Shixiong Yang, Chunmei Zeng, Qingdong Zhu   +9 more
core   +3 more sources

Third Generation Sequencing Transforming Plant Genome Research: Current Trends and Challenges

, 2023
Supplementary dataset for the review article "Third Generation Sequencing Transforming Plant Genome Research: Current Trends and Challenges" listing out the plant genomes sequenced through First generation, Second generation, Third Generation ...
Kalita, Eeshan, Kalita, E (via Mendeley Data)   +1 more
core   +3 more sources

Application of third-generation sequencing to herbal genomics

Frontiers in Plant Science, 2023
There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have
Longlong Gao, Wenjie Xu, Tianyi Xin, Jingyuan Song   +3 more
doaj   +1 more source

Third‐Generation Sequencing of Epigenetic DNA [PDF]

, 2023
The discovery of epigenetic bases has revolutionised the understanding of disease and development. Among the most studied epigenetic marks are cytosines covalently modified at the 5 position.
Bethany Searle, Andrew Kellett, Kellett, Andrew, Müller, Markus, Thomas Carell, Carell, Thomas, Markus Müller, Searle, Bethany   +7 more
core   +1 more source

Advances and Trends in Omics Technology Development

Frontiers in Medicine, 2022
The human history has witnessed the rapid development of technologies such as high-throughput sequencing and mass spectrometry that led to the concept of “omics” and methodological advancement in systematically interrogating a cellular system.
Xiaofeng Dai, Li Shen
doaj   +1 more source

Application of third-generation sequencing in cancer research

Medical Review, 2021
In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
doaj   +1 more source

Identification of rare thalassemia variants using third-generation sequencing

Frontiers in Genetics, 2023
Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu, Qianting Chen, Zonglei Zhang, Shiyi Peng, Jing Liu, Jialun Pang, Zhengjun Jia, Hui Xi, Jiaqi Li, Libao Chen, Yinyin Liu, Ying Peng   +11 more
doaj   +1 more source

SequelTools: a suite of tools for working with PacBio Sequel raw sequence data

BMC Bioinformatics, 2020
Background PacBio sequencing is an incredibly valuable third-generation DNA sequencing method due to very long read lengths, ability to detect methylated bases, and its real-time sequencing methodology.
David E. Hufnagel, Matthew B. Hufford, Arun S. Seetharam   +2 more
doaj   +1 more source

MASQC: Next Generation Sequencing Assists Third Generation Sequencing for Quality Control in N6-Methyladenine DNA Identification

Frontiers in Genetics, 2020
DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang, Yaoxin Wang, Ying Chen, Qi Dai   +3 more
doaj   +1 more source