Results 1 to 10 of about 6,398,476 (257)

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
Syahzuwan Hassan, Rosnah Bahar, Muhammad Farid Johan, Ezzeddin Kamil Mohamed Hashim, Wan Zaidah Abdullah, Ezalia Esa, Faidatul Syazlin Abdul Hamid, Zefarina Zulkafli   +7 more
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Current research status of third-generation sequencing technology in thalassemia detection [PDF]

Frontiers in Pediatrics
Thalassemia is a hereditary hemolytic disorder primarily caused by defects in the hemoglobin genes, which impede the synthesis of hemoglobin peptide chains. This disease is mainly classified into two types: α and β.
Fenglin Zhu, Fenglin Zhu, Yunli Lai, Sheng He   +3 more
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The Third-Generation Sequencing Challenge: Novel Insights for the Omic Sciences [PDF]

Biomolecules
The understanding of the human genome has been greatly improved by the advent of next-generation sequencing technologies (NGS). Despite the undeniable advantages responsible for their widespread diffusion, these methods have some constraints, mainly ...
Carmela Scarano, Iolanda Veneruso, Rosa Redenta De Simone, Gennaro Di Bonito, Angela Secondino, Valeria D’Argenio   +5 more
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Application of third-generation sequencing to herbal genomics

Frontiers in Plant Science, 2023
There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have
Longlong Gao, Wenjie Xu, Tianyi Xin, Jingyuan Song   +3 more
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Advances and Trends in Omics Technology Development

Frontiers in Medicine, 2022
The human history has witnessed the rapid development of technologies such as high-throughput sequencing and mass spectrometry that led to the concept of “omics” and methodological advancement in systematically interrogating a cellular system.
Xiaofeng Dai, Li Shen
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Application of third-generation sequencing in cancer research

Medical Review, 2021
In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
doaj   +1 more source

Identification of rare thalassemia variants using third-generation sequencing

Frontiers in Genetics, 2023
Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu, Qianting Chen, Zonglei Zhang, Shiyi Peng, Jing Liu, Jialun Pang, Zhengjun Jia, Hui Xi, Jiaqi Li, Libao Chen, Yinyin Liu, Ying Peng   +11 more
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SequelTools: a suite of tools for working with PacBio Sequel raw sequence data

BMC Bioinformatics, 2020
Background PacBio sequencing is an incredibly valuable third-generation DNA sequencing method due to very long read lengths, ability to detect methylated bases, and its real-time sequencing methodology.
David E. Hufnagel, Matthew B. Hufford, Arun S. Seetharam   +2 more
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MASQC: Next Generation Sequencing Assists Third Generation Sequencing for Quality Control in N6-Methyladenine DNA Identification

Frontiers in Genetics, 2020
DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang, Yaoxin Wang, Ying Chen, Qi Dai   +3 more
doaj   +1 more source

#201 : Comparison of the Sensitivity of Detecting Cervical Bacteria with Next Generation Sequencing and Third Generation Sequencing Technologies

Fertility & Reproduction, 2023
Background and Aims: In in vitro fertilization (IVF) cycles, some patients suffering with vaginosis showed poor reproductive outcome even transferring with good quality embryos.
Wei-Fang Chang, Shu-Min Chang, Pei-Ling Chu, Yi-Hsiu Chen, Rui-Hua Lee, Yi-Xuan Lee, Shun-Jen Tan, Ruey-Sheng Wang, Jason Yen-Ping Ho, Shyr-Yeu Lin, Chii-Ruey Tzeng, Yu-Ming Hu   +11 more
doaj   +1 more source