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Application of third-generation sequencing to herbal genomics [PDF]

Frontiers in Plant Science, 2023
There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have
Longlong Gao, Wenjie Xu, Tianyi Xin, Jingyuan Song   +3 more
doaj   +4 more sources

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
Syahzuwan Hassan, Rosnah Bahar, Muhammad Farid Johan, Ezzeddin Kamil Mohamed Hashim, Wan Zaidah Abdullah, Ezalia Esa, Faidatul Syazlin Abdul Hamid, Zefarina Zulkafli   +7 more
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Application of third-generation sequencing in cancer research [PDF]

Medical Review, 2021
In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
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Identification of rare thalassemia variants using third-generation sequencing

Frontiers in Genetics, 2023
Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu, Qianting Chen, Zonglei Zhang, Shiyi Peng, Jing Liu, Jialun Pang, Zhengjun Jia, Hui Xi, Jiaqi Li, Libao Chen, Yinyin Liu, Ying Peng   +11 more
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MASQC: Next Generation Sequencing Assists Third Generation Sequencing for Quality Control in N6-Methyladenine DNA Identification [PDF]

Frontiers in Genetics, 2020
DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang, Yaoxin Wang, Ying Chen, Qi Dai   +3 more
doaj   +3 more sources

Viral oncogenes, viruses, and cancer: a third-generation sequencing perspective on viral integration into the human genome [PDF]

Frontiers in Oncology, 2023
The link between viruses and cancer has intrigued scientists for decades. Certain viruses have been shown to be vital in the development of various cancers by integrating viral DNA into the host genome and activating viral oncogenes.
Ruichen Ye, Ruichen Ye, Ruichen Ye, Angelina Wang, Brady Bu, Pengxiang Luo, Wenjun Deng, Xinyi Zhang, Shanye Yin, Shanye Yin   +9 more
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Application of third-generation sequencing technology for identifying rare α- and β-globin gene variants in a Southeast Chinese region [PDF]

BMC Medical Genomics
Background Third-generation sequencing (TGS) based on long-read technology has been gradually used in identifying thalassemia and hemoglobin (Hb) variants.
Jianlong Zhuang, Junyu Wang, Nan Huang, Yu Zheng, Liangpu Xu   +4 more
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The Third-Generation Sequencing Challenge: Novel Insights for the Omic Sciences [PDF]

Biomolecules
The understanding of the human genome has been greatly improved by the advent of next-generation sequencing technologies (NGS). Despite the undeniable advantages responsible for their widespread diffusion, these methods have some constraints, mainly ...
Carmela Scarano, Iolanda Veneruso, Rosa Redenta De Simone, Gennaro Di Bonito, Angela Secondino, Valeria D’Argenio   +5 more
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Advances and Trends in Omics Technology Development

Frontiers in Medicine, 2022
The human history has witnessed the rapid development of technologies such as high-throughput sequencing and mass spectrometry that led to the concept of “omics” and methodological advancement in systematically interrogating a cellular system.
Xiaofeng Dai, Li Shen
doaj   +1 more source

SequelTools: a suite of tools for working with PacBio Sequel raw sequence data

BMC Bioinformatics, 2020
Background PacBio sequencing is an incredibly valuable third-generation DNA sequencing method due to very long read lengths, ability to detect methylated bases, and its real-time sequencing methodology.
David E. Hufnagel, Matthew B. Hufford, Arun S. Seetharam   +2 more
doaj   +1 more source