New technologies accelerate the exploration of non-coding RNAs in horticultural plants. [PDF]
, 2017 Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small ...
Hu, Rongbin +4 more
core +3 more sources
An Overview of the Second and Third-Generation of DNA Sequencing Technologies [PDF]
Journal of Advanced Biomedical Sciences, 2017 DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid.
Fatemeh Ghorbani Parsa +1 more
doaj
ForestQC: Quality control on genetic variants from next-generation sequencing data using random forest. [PDF]
, 2019 Next-generation sequencing technology (NGS) enables the discovery of nearly all genetic variants present in a genome. A subset of these variants, however, may have poor sequencing quality due to limitations in NGS or variant callers.
Coppola, Giovanni +6 more
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Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report
Frontiers in Cellular and Infection Microbiology, 2023 Mycobacterium marinum (M. marinum) is a non-tuberculous mycobacterium (NTM) that can cause infectious diseases in aquatic animals and humans. Culture-based pathogen detection is the gold standard for diagnosing NTM infection. However, this method is time-
Yan-Ying Huang +4 more
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Comparison of TCGA and GENIE genomic datasets for the detection of clinically actionable alterations in breast cancer. [PDF]
, 2019 Whole exome sequencing (WES), targeted gene panel sequencing and single nucleotide polymorphism (SNP) arrays are increasingly used for the identification of actionable alterations that are critical to cancer care.
Carpten, John D +4 more
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Advances in Integrating Genomics and Bioinformatics in the Plant Breeding Pipeline
Agriculture, 2018 With the global human population growing rapidly, agricultural production must increase to meet crop demand. Improving crops through breeding is a sustainable approach to increase yield and yield stability without intensifying the use of fertilisers and ...
Haifei Hu, Armin Scheben, David Edwards
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Life, 2022 Next-generation sequencing (NGS) applications have flourished in the last decade, permitting the identification of cancer driver genes and profoundly expanding the possibilities of genomic studies of cancer, including melanoma. Here we aimed to present a
Adrián Muñoz-Barrera +7 more
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Emerging Microbes and Infections, 2023 Virus circular RNAs (circRNA) have been reported to be extensively expressed and play important roles in viral infections. Previously we build the first database of virus circRNAs named VirusCircBase which has been widely used in the field.
Ping Fu +4 more
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Evaluation of experimental design and computational parameter choices affecting analyses of ChIP-seq and RNA-seq data in undomesticated poplar trees. [PDF]
, 2014 BackgroundOne of the great advantages of next generation sequencing is the ability to generate large genomic datasets for virtually all species, including non-model organisms.
Filkov, Vladimir +4 more
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NGSpeciesID: DNA barcode and amplicon consensus generation from long‐read sequencing data
Ecology and Evolution, 2021 Third‐generation sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have gained popularity over the last years. These platforms can generate millions of long‐read sequences. This is not only advantageous
Kristoffer Sahlin +2 more
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