Results 41 to 50 of about 6,398,476 (257)
Evolutionary Insight into the Trypanosomatidae Using Alignment-Free Phylogenomics of the Kinetoplast
Pathogens, 2019 Advancements in next-generation sequencing techniques have led to a substantial increase in the genomic information available for analyses in evolutionary biology.
Alexa Kaufer, Damien Stark, John Ellis
doaj +1 more source
Long walk to genomics: History and current approaches to genome sequencing and assembly
Computational and Structural Biotechnology Journal, 2020 Genomes represent the starting point of genetic studies. Since the discovery of DNA structure, scientists have devoted great efforts to determine their sequence in an exact way.
Alice Maria Giani +3 more
doaj +1 more source
Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future? [PDF]
, 2012 Hepatocellular Carcinoma (HCC) is the third most deadly malignancy worldwide characterized by phenotypic and molecular heterogeneity. In the past two decades, advances in genomic analyses have formed a comprehensive understanding of different underlying ...
Marquardt, Jens U +3 more
core +1 more source
NGSpeciesID: DNA barcode and amplicon consensus generation from long‐read sequencing data
Ecology and Evolution, 2021 Third‐generation sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have gained popularity over the last years. These platforms can generate millions of long‐read sequences. This is not only advantageous
Kristoffer Sahlin +2 more
doaj +1 more source
Improvement of large copy number variant detection by whole genome nanopore sequencing
Journal of Advanced Research, 2023 Introduction: Whole-genome sequencing using nanopore technologies can uncover structural variants, which are DNA rearrangements larger than 50 base pairs.
Javier Cuenca-Guardiola +5 more
doaj +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
Explore of nanopore sequencing technology in ambiguities of HLA genotyping
Zhongguo shuxue zazhi[Objective] To resolve the ambiguities of HLA genotyping generated by next generation sequencing (NGS) using nanopore sequencing technology. [Methods] A total of 38 samples with ambiguous HLA genotyping by NGS in our laboratory were collected, and HLA-A,
CHEN Nanying +6 more
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
, 2021 Among all economically important plant species in the world, grapevine (Vitis vinifera L.) is the most cultivated fruit plant. It has a significant impact on the economies of many countries through wine and fresh and dried fruit production.
Charith Raj Adkar-Purushothama +5 more
core +1 more source