Results 31 to 40 of about 6,398,476 (257)

Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report

Frontiers in Cellular and Infection Microbiology, 2023
Mycobacterium marinum (M. marinum) is a non-tuberculous mycobacterium (NTM) that can cause infectious diseases in aquatic animals and humans. Culture-based pathogen detection is the gold standard for diagnosing NTM infection. However, this method is time-
Yan-Ying Huang, Qiu-Shi Li, Zhao-Dong Li, Ai-Hua Sun, Sheng-Ping Hu   +4 more
doaj   +1 more source

An Overview of the Second and Third-Generation of DNA Sequencing Technologies [PDF]

Journal of Advanced Biomedical Sciences, 2017
DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid.
Fatemeh Ghorbani Parsa, Mohammad Ali Hossein Pour Feizi   +1 more
doaj  

Advances in Integrating Genomics and Bioinformatics in the Plant Breeding Pipeline

Agriculture, 2018
With the global human population growing rapidly, agricultural production must increase to meet crop demand. Improving crops through breeding is a sustainable approach to increase yield and yield stability without intensifying the use of fertilisers and ...
Haifei Hu, Armin Scheben, David Edwards
doaj   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Krüger Detlev H., Kentaro Yoshida, Kahl, G., Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Yoshida Kentaro, Luo, S., Shujun Luo, Barrero, R.A., Schroth, Gary P., Detlev H Krüger, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Kahl Günter, Luo Shujun, Albertyn Zayed, Gary P. Schroth, Matsumura, Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Matsumura Hideo, Barrero Roberto A., Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Roberto A. Barrero, Günter Kahl, Kahl, Guenter, Schroth, Gary P, Krueger, Detlev H, Barrero, Roberto A   +50 more
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An updated database of virus circular RNAs provides new insights into the biogenesis mechanism of the molecule

Emerging Microbes and Infections, 2023
Virus circular RNAs (circRNA) have been reported to be extensively expressed and play important roles in viral infections. Previously we build the first database of virus circRNAs named VirusCircBase which has been widely used in the field.
Ping Fu, Zena Cai, Zhiyuan Zhang, Xiangxian Meng, Yousong Peng   +4 more
doaj   +1 more source

From Samples to Germline and Somatic Sequence Variation: A Focus on Next-Generation Sequencing in Melanoma Research

Life, 2022
Next-generation sequencing (NGS) applications have flourished in the last decade, permitting the identification of cancer driver genes and profoundly expanding the possibilities of genomic studies of cancer, including melanoma. Here we aimed to present a
Adrián Muñoz-Barrera, Luis A. Rubio-Rodríguez, Ana Díaz-de Usera, David Jáspez, José M. Lorenzo-Salazar, Rafaela González-Montelongo, Víctor García-Olivares, Carlos Flores   +7 more
doaj   +1 more source

Moving towards third-generation sequencing technologies

, 2012
Within just the past few years, next-generation sequencing (NGS) platforms have reduced the cost of DNA sequencing by several orders of magnitude. With these lower costs, DNA sequencing is increasingly powerful as a single-data format onto which a broad ...
Janitz, Michael, Janitz, Caroline (R17566), Karolina Janitz, Michal Janitz   +3 more
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Application of second-generation sequencing (SGS) and third generation sequencing (TGS) in aquaculture breeding program

, 2022
High-throughput sequencing technologies have expanded gene-based to genome-wide research in aquaculture species. Several techniques adopting the Second Generation Sequencing (SGS), Third Generation Sequencing (TGS) platforms and/or hybrid genome ...
Sung, Yeong Yik, Asaduzzaman, Md, Nor, Siti Azizah Mohd, Van de Peer, Yves, Ma, Hongyu, Liang Yantao, Liang Yantao, Razali, Siti Aisyah, Deris, Zulaikha Mat, Sorgeloos, Patrick, Danish-Daniel, Muhd, Liu, Zhanjiang, Tan, Min Pau, Afiqah-Aleng, Nor, Min, Wang, Wong, Li Lian, Li Lian Wong, Wang Min, Md Asaduzzaman, Yeong Yik Sung, Min Pau Tan, Zulaikha Mat Deris, Siti Aisyah Razali, Patrick Sorgeloos, Siti Azizah Mohd Nor, Hongyu Ma, Liang Yantao, Zhanjiang Liu, Muhd Danish-Daniel, Nor Afiqah-Aleng, Yves Van de Peer   +29 more
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A hybrid correcting method considering heterozygous variations by a comprehensive probabilistic model

BMC Genomics, 2020
Background The emergence of the third generation sequencing technology, featuring longer read lengths, has demonstrated great advancement compared to the next generation sequencing technology and greatly promoted the biological research.
Jiaqi Liu, Jiayin Wang, Xiao Xiao, Xin Lai, Daocheng Dai, Xuanping Zhang, Xiaoyan Zhu, Zhongmeng Zhao, Juan Wang, Zhimin Li   +9 more
doaj   +1 more source

Analyzing Modern Biomolecules: The Revolution of Nucleic-Acid Sequencing – Review

Biomolecules, 2021
Recent developments have revolutionized the study of biomolecules. Among them are molecular markers, amplification and sequencing of nucleic acids. The latter is classified into three generations. The first allows to sequence small DNA fragments.
Gabriel Dorado, Sergio Gálvez, Teresa E. Rosales, Víctor F. Vásquez, Pilar Hernández   +4 more
doaj   +1 more source