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PacBio Sequencing and its Applications
Genomics, Proteomics and Bioinformatics, 2015 Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read lengths than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research ...
Anthony Rhoads, Kin Fai Au
exaly +5 more sources
PacBio Long Reads Improve Metagenomic Assemblies, Gene Catalogs, and Genome Binning
Frontiers in Genetics, 2020 PacBio long reads sequencing presents several potential advantages for DNA assembly, including being able to provide more complete gene profiling of metagenomic samples.
Haiying Xie +7 more
exaly +3 more sources
Plant Methods, 2020 Background The number of studies using third-generation sequencing utilising Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas.
Zhaogeng Lu, Biao Jin
exaly +3 more sources
Genome-wide methylation detection and episignature analysis using PacBio long-read sequencing [PDF]
Genome MedicineBackground The detection of 5-methylcytosine (5mC) patterns in the human genome is relevant for the diagnosis of various genetic conditions. Genome-wide methylation episignatures provide a new approach for resolving variants of uncertain significance ...
Véronique Ivashchenko +14 more
doaj +2 more sources
Frontiers in Microbiology, 2017 Although PacBio third-generation sequencers have improved the read lengths of genome sequencing which facilitates the assembly of complete genomes, no study has reported success in using PacBio data alone to completely sequence a two-chromosome bacterial
Jade L L Teng, Elaine Chan, Lilong Jia
exaly +3 more sources
Mitochondrial DNA. Part B. Resources, 2023 The complete mitogenome of the Atlantic spiny lumpsucker (Eumicrotremus spinosus) was generated using the PacBio Sequel II HiFi sequencing platform. The mitogenome assembly has a length of 19,281 bp and contains 13 protein-coding sequences, 22 tRNA genes,
Likith R. Pinninti +6 more
doaj +1 more source
Frontiers in Genetics, 2021 Background and Objectives: Castor (Ricinus communis L.) is an important non-edible oilseed crop. Lm-type female strains and normal amphiprotic strains are important castor cultivars, and are mainly different in their inflorescence structures and leaf ...
Yaxing Zhou +4 more
doaj +1 more source
Comprehensive profiling of rhizome-associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis). [PDF]
, 2017 Moso bamboo (Phyllostachys edulis) represents one of the fastest-spreading plants in the world, due in part to its well-developed rhizome system. However, the post-transcriptional mechanism for the development of the rhizome system in bamboo has not been
Cai, Dawei +8 more
core +1 more source
Large-scale and significant expression from pseudogenes in Sodalis glossinidius – a facultative bacterial endosymbiont [PDF]
, 2020 The majority of bacterial genomes have high coding efficiencies, but there are some genomes of intracellular bacteria that have low gene density. The genome of the endosymbiont Sodalis glossinidius contains almost 50 % pseudogenes containing mutations ...
Beynon, R +9 more
core +2 more sources
Complete high-quality genome sequence of Clostridium limosum (Hathewaya limosa) isolate 14S0207, recovered from a cow with suspected blackleg in Germany [PDF]
, 2020 Clostridium limosum can be found in soil and the intestinal tract of animals. In 2014, C. limosum was isolated from a suspected blackleg outbreak in cattle in Schleswig-Holstein, Germany. We present a complete genome sequence of a C.
Abdel-Glil, Mostafa Y. +7 more
core +1 more source